Recombinant polypeptide antigen-based immunoglobulin G enzyme-linked immunosorbent assay for serodiagnosis of dengue

被引：6

作者：

Dos Santos, Flavia B.

Nogeira, Rita Maria R.

Lima, Monique R. Q.

De Simone, Thatiane S.

Schatzmayr, Hermann G.

Lemes, Elezer A. B.

Harris, Eva

Miagostovich, Marize P.

机构：

[1] Fiocruz MS, Inst Oswaldo Cruz, Flavivirus Lab, Dept Virol, BR-21045360 Rio De Janeiro, Brazil

[2] Dept Technol Dev, BR-21045360 Rio De Janeiro, Brazil

[3] Univ Calif Berkeley, Sch Publ Hlth, Div Infect Dis, Berkeley, CA 94720 USA

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2007年 / 14卷 / 05期

关键词：

D O I：

10.1128/CVI.00474-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.

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页码：641 / 643

页数：3

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