Association of a phloem-limited bacterium with yellow vine disease in cucurbits

被引:36
作者
Bruton, BD [1 ]
Fletcher, J
Pair, SD
Shaw, M
Sittertz-Bhatkar, H
机构
[1] USDA, ARS, Lane, OK 74555 USA
[2] Oklahoma State Univ, Dept Plant Pathol, Stillwater, OK 74078 USA
[3] Texas A&M Univ, Ctr Electron Microscopy, College Stn, TX 77843 USA
关键词
Citrullus lanatus; Cucumis melo; Cucurbita spp; insect; vine decline;
D O I
10.1094/PDIS.1998.82.5.512
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Since 1991, a new disease of cucurbits in central Texas and Oklahoma, designated yellow vine, has resulted in the decline and plant death of watermelon, cantaloupe. squash, and pumpkin. Affected plants are characterized by leaf yellowing, phloem discoloration, and plant collapse. Year-to-year variation in disease incidence has ranged from spotty outbreaks to complete crop loss in early-planted watermelon fields. A systematic investigation to determine the causal agent of the disease included pathogen isolation attempts, transmission tests, serological assays with various antisera (enzyme-linked immunosorbent assay and Western blotting), and DNA hybridizations with selected probes (dot and Southern blots). None of these tests revealed a consistent relationship between the expression of yellow vine symptoms and the presence of a particular microorganism or virus in the plant. However, transmission electron microscopic examination showed the consistent presence of a bacterium in the phloem sieve elements of symptomatic plants. The rod-shaped bacteria, observed only in symptomatic cucurbits, measured 0.25 to 0.5 mu m in width and 1.0 to 3.0 mu m in length and were surrounded by a triple-layered cell envelope.
引用
收藏
页码:512 / 520
页数:9
相关论文
共 45 条
  • [1] AUSTIN ME, 1965, USDA SOIL CONSERVATO, V296
  • [2] AUSUBAL FM, 1987, CURRENT PROTOCOLS MO
  • [3] AVILA FJ, IN PRESS PHYTOPATHOL
  • [4] Bove J. M., 1974, Proceedings of the Sixth Conference of the International Organization of Citrus Virologists., P12
  • [5] BRUTON BD, 1996, COMPENDIUM CUCURBIT, P10
  • [6] BRUTON BD, 1995, P CUC 94, P151
  • [7] BRUTON BD, 1988, TEXAS AGR EXT SERV B, V1595
  • [8] BRUTON BD, 1995, USDA PUBL SCARL, V101
  • [9] BRUTON BD, PLANT SCI, V47, P53
  • [10] CHARACTERISTICS OF MICROPLATE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF PLANT-VIRUSES
    CLARK, MF
    ADAMS, AN
    [J]. JOURNAL OF GENERAL VIROLOGY, 1977, 34 (MAR) : 475 - 483