Long-term starvation survival of a thermophilic sulfidogen consortium

被引:6
作者
Bass, CJ [1 ]
Davey, RA [1 ]
Lappin-Scott, HM [1 ]
机构
[1] Univ Exeter, Dept Biol Sci, Exeter EX4 4PS, Devon, England
关键词
filterable bacteria; nutrient deprivation; sulfate-reducing bacteria; sulfidogen; survival; thermophilic; ultramicrobacteria;
D O I
10.1080/01490459809378060
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A bacterial consortium containing thermophilic sulfidogens was obtained from filtration of produced fluids from a North Sea oil production facility. It was subjected to two distinct starvation regimes considered to be representative of those that might be experienced by such organisms surviving either (a) in open seawater prior to injection into the formation with secondary recovery fluids, or (b) in secondary recovery water-floods deep in the reservoir. Metabolic activity measurements and resuscitation data together with scanning electron microscopy indicate survival for 21 months with no available carbon source. Survival was measured by the starved cells' ability to reduce intracellularly the metabolic indicator INT to INT-formazan. By this method, starvation survival was demonstrated in all samples tested over the experimental period (up to 21 months). The indication of such ability was not consistently accompanied by resuscitation and growth in media previously used for culture maintenance and propagation.
引用
收藏
页码:29 / 36
页数:8
相关论文
共 18 条
[1]   Influence of surfaces on sulphidogenic bacteria [J].
Bass, CJ ;
Webb, JS ;
Sanders, PF ;
LappinScott, HM .
BIOFOULING, 1996, 10 (1-3) :95-109
[2]  
BASS CJ, 1993, J OFFSHORE TECHNOL, V1, P31
[3]   TETRAZOLIUM REDUCTION MALACHITE GREEN METHOD FOR ASSESSING THE VIABILITY OF FILAMENTOUS BACTERIA IN ACTIVATED-SLUDGE [J].
BITTON, G ;
KOOPMAN, B .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 43 (04) :964-966
[4]   THERMOPHILIC BACTERIA AMONG ARCTIC, SUB-ARCTIC, AND ALPINE HABITATS [J].
BOYD, WL ;
ONN, DR ;
BOYD, JW .
ARCTIC AND ALPINE RESEARCH, 1990, 22 (04) :401-411
[5]   Survival of parental and genetically modified derivatives of a soil isolated Pseudomonas fluorescens under nutrient-limiting conditions [J].
Clegg, CD ;
vanElsas, JD ;
Anderson, JM ;
LappinScott, HM .
JOURNAL OF APPLIED BACTERIOLOGY, 1996, 81 (01) :19-26
[6]  
KAPRELYANTS AS, 1993, FEMS MICROBIOL LETT, V104, P271, DOI 10.1111/j.1574-6968.1993.tb05871.x
[7]   THE TRANSIENT PHASE BETWEEN GROWTH AND NONGROWTH OF HETEROTROPHIC BACTERIA, WITH EMPHASIS ON THE MARINE-ENVIRONMENT [J].
KJELLEBERG, S ;
HERMANSSON, M ;
MARDEN, P .
ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :25-49
[8]   SURVIVAL MECHANISMS OF HYDROGEN SULFIDE-PRODUCING BACTERIA ISOLATED FROM EXTREME ENVIRONMENTS AND THEIR ROLE IN CORROSION [J].
LAPPINSCOTT, HM ;
BASS, CJ ;
MCALPINE, K ;
SANDERS, PF .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 1994, 34 (3-4) :305-319
[9]   ISOLATION AND CHARACTERIZATION OF ULTRA-MICROBACTERIA FROM A GULF-COAST ESTUARY [J].
MACDONELL, MT ;
HOOD, MA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 43 (03) :566-571
[10]   THE STARVATION-SURVIVAL STATE OF MICROORGANISMS IN NATURE AND ITS RELATIONSHIP TO THE BIOAVAILABLE ENERGY [J].
MORITA, RY .
EXPERIENTIA, 1990, 46 (08) :813-817