Effects of N-Acetylcysteine/Deferoxamine, Taurine and RC-3095 on Respiratory Chain Complexes and Creatine Kinase Activities in Rat Brain After Sepsis

被引：29

作者：

Cassol, Omar J., Jr. ^{[1
]}

Rezin, Gislaine T. ^{[1
]}

Petronilho, Fabricia C. ^{[1
]}

Scaini, Giselli ^{[1
]}

Goncalves, Cinara L. ^{[1
]}

Ferreira, Gabriela K. ^{[1
]}

Roesler, Rafael ^{[2
,3
]}

Schwartsmann, Gilberto ^{[3
]}

Dal-Pizzol, Felipe ^{[1
]}

Streck, Emilio L. ^{[1
]}

机构：

[1] Univ Extremo Catarinense, Lab Fisiopatol Expt, Programa Posgrad Ciencias Saude, BR-88806000 Criciuma, SC, Brazil

[2] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Farmacol, Grp Pesquisa Neurofarmacol Celular & Mol, BR-90046900 Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Hosp Clin Porto Alegre, Lab Pesquisas Canc, BR-90035003 Porto Alegre, RS, Brazil

来源：

NEUROCHEMICAL RESEARCH | 2010年 / 35卷 / 04期

关键词：

Sepsis; Mitochondrial respiratory chain; Creatine kinase; Taurine; RC-3095; GASTRIN-RELEASING-PEPTIDE; RECEPTOR ANTAGONIST RC-3095; ALZHEIMERS-DISEASE BRAIN; OXIDATIVE STRESS; CECAL LIGATION; MITOCHONDRIAL DYSFUNCTION; COGNITIVE IMPAIRMENT; ENERGY-METABOLISM; PLUS DEFEROXAMINE; PROTECTS RATS;

D O I：

10.1007/s11064-009-0089-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The pathogenesis of sepsis is characterized by an overwhelming systemic inflammatory response that can lead to multiple organ failure. Considering that we have recently demonstrated that mitochondrial respiratory chain and creatine kinase (CK) are altered in the brain of rats after cecal ligation and perforation (CLP) and that a combination of N-acetylcysteine/deferoxamine (NAC/DFX), taurine and RC-3095 were shown to be an effective treatment of sepsis, we investigated whether the alterations of these enzymes may be reversed by these drugs. The results demonstrated that CLP inhibited complexes I and II, and that all the treatments were able to reverse this inhibition in all brain areas studied in the present work. On the other hand, complexes III and IV were not affected by sepsis neither by any of the treatments. An increase in CK activity in brain of rats 12 h after CLP was also verified; the administration of NAC/DFX and taurine reversed the increase in CK activity in hippocampus, cerebral cortex, cerebellum and striatum. On the other hand, RC-3095 significantly decreased CK activity, when compared to sham group in all brain areas studied. This is a preliminary study which showed beneficial effects of the treatments we proposed.

引用

页码：515 / 521

页数：7

共 58 条

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Quercetin, coenzyme Q10 and L-canavanine as protective agents against lipid peroxidation and nitric oxide generation in endotoxin-induced shock in rat brain [J].