An Escherichia coli hydrogenase-3-type hydrogenase in methanogenic archaea

被引:86
作者
Künkel, A
Vorholt, JA
Thauer, RK
Hedderich, R
机构
[1] Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany
[2] Univ Marburg, Mikrobiol Lab, Fachbereich Biol, Marburg, Germany
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 252卷 / 03期
关键词
hydrogenase; Escherichia coli hydrogenase 3; NADH : ubiquinone oxidoreductase; energy conservation; Methanosarcina barkeri; methanogenic Archaea;
D O I
10.1046/j.1432-1327.1998.2520467.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Methanogenic archaea are known to contain two types of [NiFe] hydrogenases designated F(420)-reducing hydrogenase and F(420)-non-reducing hydrogenase. We report here that they additionally contain Escherichia coli hydrogenase-3-type [NiFe] hydrogenases. The evidence is based on biochemical studies and analysis of the subunit primary structure of this hydrogenase (designated Ech) purified from membranes of acetate-grown cells of Methanosarcina barkeri. The subunits EchE and EchC of the EchABCDEF complex showed 34% and 45% sequence identity to the nickel-containing large subunit HycE and to the iron-sulfur cluster containing small subunit HycG, respectively, of the hydrogenase in the formate hydrogen lyase complex from E. coli. Analysis of the totally sequenced genomes of Methanococcus jannaschii and Methanobacterium thermoautotrophicum strain Delta H revealed that these organisms contain similar open reading frames, indicating the presence of an E. coli hydrogenase-3-type hydrogenase also in these methanogenic archaea.
引用
收藏
页码:467 / 476
页数:10
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