Asp-285 of the metal-tetracycline/H+ antiporter of Escherichia coli is essential for substrate binding

被引：17

作者：

Kimura, T ^{[1
]}

Yamaguchi, A ^{[1
]}

机构：

[1] OSAKA UNIV,INST SCI & IND RES,DEPT CELL MEMBRANE BIOL,IBARAKI,OSAKA 567,JAPAN

来源：

FEBS LETTERS | 1996年 / 388卷 / 01期

关键词：

tetracycline; tetracycline/H+ antiporter; Tet; photolabel; substrate binding;

D O I：

10.1016/0014-5793(96)00514-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transposon Tn10-encoded metal-tetracycline/H+ antiporter (TetA(B)) was preferentially photolabeled when [H-3]tetracycline was irradiated in the presence of energized inverted membrane vesicles containing the TetA protein, The degree of labeling depended on the duration of irradiation and the energization of the membrane. Photolabeling was not observed in vesicles containing the Asp-285 --> Asn mutant TetA protein, indicating that Asp-285 participates in the substrate binding or the step(s) prior to substrate binding

引用

页码：50 / 52

页数：3

共 23 条

[1]

BRUGGEMANN EP, 1992, J BIOL CHEM, V267, P21020

[2] PHOTOINCORPORATION OF TETRACYCLINE INTO ESCHERICHIA-COLI RIBOSOMES - IDENTIFICATION OF LABELED PROTEINS AND FUNCTIONAL CONSEQUENCES [J].

GOLDMAN, RA ;

COOPERMAN, BS ;

STRYCHARZ, WA ;

WILLIAMS, BA ;

TRITTON, TR .

FEBS LETTERS, 1980, 118 (01) :113-122

[3] PHOTOINCORPORATION OF TETRACYCLINE INTO ESCHERICHIA-COLI RIBOSOMES - IDENTIFICATION OF THE MAJOR PROTEINS PHOTOLABELED BY NATIVE TETRACYCLINE AND TETRACYCLINE PHOTOPRODUCTS AND IMPLICATIONS FOR THE INHIBITORY-ACTION OF TETRACYCLINE ON PROTEIN-SYNTHESIS [J].

GOLDMAN, RA ;

HASAN, T ;

HALL, CC ;

STRYCHARZ, WA ;

COOPERMAN, BS .

BIOCHEMISTRY, 1983, 22 (02) :359-368

[4]

GREENBERGER LM, 1993, J BIOL CHEM, V268, P11417

[5] PROTON-LINKED SUGAR-TRANSPORT SYSTEMS IN BACTERIA [J].

HENDERSON, PJF .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1990, 22 (04) :525-569

[6] SUBSTRATE-INDUCED ACCELERATION OF N-ETHYLMALEIMIDE REACTION WITH THE CYS-65 MUTANT OF THE TRANSPOSON TN10-ENCODED METAL-TETRACYCLINE H+ ANTIPORTER DEPENDS ON THE INTERACTION OF ASP-66 WITH THE SUBSTRATE [J].

KIMURA, T ;

INAGAKI, Y ;

SAWAI, T ;

YAMAGUCHI, A .

FEBS LETTERS, 1995, 362 (01) :47-49

[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[8] MULTICOPY TN10 TET PLASMIDS CONFER SENSITIVITY TO INDUCTION OF TET GENE-EXPRESSION [J].

MOYED, HS ;

NGUYEN, TT ;

BERTRAND, KP .

JOURNAL OF BACTERIOLOGY, 1983, 155 (02) :549-556

[9] EFFLUX-MEDIATED MULTIDRUG RESISTANCE IN BACILLUS-SUBTILIS - SIMILARITIES AND DISSIMILARITIES WITH THE MAMMALIAN SYSTEM [J].

NEYFAKH, AA ;

BIDNENKO, VE ;

CHEN, LB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4781-4785

[10] TRANSLATION OF MULV AND MSV RNAS IN NUCLEASE-TREATED RETICULOCYTE EXTRACTS - ENHANCEMENT OF GAG-POL POLYPEPTIDE WITH YEAST SUPPRESSOR TRANSFER-RNA [J].

PHILIPSON, L ;

ANDERSSON, P ;

OLSHEVSKY, U ;

WEINBERG, R ;

BALTIMORE, D ;

GESTELAND, R .

CELL, 1978, 13 (01) :189-199

← 1 2 3 →