Isolation and identification of a novel Ala-Pro-Gly-Trp-amide-related peptide inhibiting the motility of the mature oviduct in the cuttlefish, Sepia officinalis

被引：41

作者：

Henry, J ^{[1
]}

Favrel, P ^{[1
]}

Boucaud-Camou, E ^{[1
]}

机构：

[1] Univ Caen, Lab Biol & Biotechnol Marines, F-14032 Caen, France

来源：

PEPTIDES | 1997年 / 18卷 / 10期

关键词：

Sepia officinalis; cephalopods; molluscs; oviduct; neuropeptides; APGW-amide;

D O I：

10.1016/S0196-9781(97)00241-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel myotropic neuropeptide was isolated from 110 optic lobes (OL) of mature females of the cuttlefish Sepia officinalis L. by mean of high performance liquid chromatography (HPLC). The peptide inhibits the motility of the oviduct by decreasing the tonus, the frequency and the amplitude of the contractions. The primary structure of the peptide was determined as Gly-Trp-NH2. This new dipeptide is closely related to the Ala-Pro-Gly-Trp-NH2 family first identified in gastropod molluscs. On the perfused oviduct, GWa appeared to be 3000 times more potent than APGW-amide. The processing of synthetic APGWa into GWa by diaminopeptidyl activity has been clearly observed in OL extract. Nevertheless, the analysis in MALDI-MS of HPLC OL fractions did not reveal any APGWa related peptides of the known : APGWa, KPGWa, RPGWa and TPGWa. GWa could be processed from a not yet identified APGWa related peptide. (C) 1997 Elsevier Science Inc.

引用

页码：1469 / 1474

页数：6

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