Rapid actin transport during cell protrusion

被引：138

作者：

Zicha, D

Dobbie, IM

Holt, MR

Monypenny, J

Soong, DYH

Gray, C

Dunn, GA

机构：

[1] Kings Coll London, Randall Ctr, London SE1 1UL, England

[2] Canc Res UK, Light Microscopy, Lincolns Inn Fields Labs, London WC2A 3PX, England

[3] Natl Univ Ireland, Dept Biochem, Genome Stabil Lab, Galway, Ireland

来源：

SCIENCE | 2003年 / 300卷 / 5616期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1126/science.1082026

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transformed rat fibroblasts expressing two variants of green fluorescent protein, each fused to beta-actin, were used to study actin dynamics during cell protrusion. The recently developed FLAP (fluorescence localization after photobleaching) method permits the tracking of one fluorophore after localized photobleaching by using the other as a colocalized reference. Here, by visualizing the ratio of bleached to total molecules, we found that actin was delivered to protruding zones of the leading edge of the cell at speeds that exceeded 5 micrometers per second. Monte Carlo modeling confirmed that this flow cannot be explained by diffusion and may involve active transport.

引用

页码：142 / 145

页数：4

共 9 条

[1] Fluorescence localization after photobleaching (FLAP): a new method for studying protein dynamics in living cells [J].