A rapid method for high throughput DNA extraction from plant material for PCR amplification

被引：54

作者：

Dilworth, E ^{[1
]}

Frey, JE ^{[1
]}

机构：

[1] Swiss Fed Res Stn Fruit Growing Viticulture & Hor, CH-8820 Wadenswil, Switzerland

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2000年 / 18卷 / 01期

关键词：

Powdery Mildew Resistance; Plant Molecular Biology; Apple Scab; Federal Research Station; Swiss Federal Research Station;

D O I：

10.1007/BF02825295

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and reliable method is described for high throughput extraction of DNA from plant material using glass beads in a fiat-bottomed microtitre plate. This: procedure is quick, inexpensive, and allows up to 96 samples to be processed in parallel. PCR products produced by the recovered DNA are consistently equivalent to those produced through traditional extraction methods.

引用

页码：61 / 64

页数：4