Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

被引：683

作者：

Sekar, RB

Periasamy, A

机构：

[1] Univ Virginia, Dept Biol, WM Keck Ctr Cellular Imaging, Charlottesville, VA 22904 USA

[2] Univ Virginia, Dept Biomed Engn, WM Keck Ctr Cellular Imaging, Charlottesville, VA 22904 USA

来源：

JOURNAL OF CELL BIOLOGY | 2003年 / 160卷 / 05期

关键词：

FRET microscopy; data analysis; cameleons; dimerization; FRET assays;

D O I：

10.1083/jcb.200210140

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.

引用

页码：629 / 633

页数：5

共 28 条

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