Tree-shaped paper strip for semiquantitative colorimetric detection of protein with self-calibration

被引：115

作者：

Wang, Wei ^{[1
,2
]}

Wu, Wen-Ya ^{[1
]}

Wang, Wei ^{[1
,2
]}

Zhu, Jun-Jie ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci MOE, Nanjing 210093, Peoples R China

[2] Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224051, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 24期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Tree-shaped paper; Colorimetric detection; Self-calibration; SILVER ENHANCEMENT;

D O I：

10.1016/j.chroma.2010.04.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper described a convenient semiquantitative method for calorimetric detection of protein with self-calibration integrated on the test strip. Hydrophilic paper was employed as microfluidic device for running calorimetric assay, tree-shaped design was developed to ensure uniform microfluidic flow for multiple branches. The approach was validated with bovine serum albumin (BSA) calorimetric detection, and colorimetric results observed by naked eyes were consistent with that from apparatus. The device could be coupled with digital transmission of images for remote monitoring system for diagnosis, food control, and environmental analysis. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：3896 / 3899

页数：4

共 13 条

[1] Low-cost printing of poly(dimethylsiloxane) barriers to define microchannels in paper [J].

Bruzewicz, Derek A. ;

Reches, Meital ;

Whitesides, George M. .

ANALYTICAL CHEMISTRY, 2008, 80 (09) :3387-3392

[2] Electrochemical Detection for Paper-Based Microfluidics [J].

Dungchai, Wijitar ;

Chailapakul, Orawon ;

Henry, Charles S. .

ANALYTICAL CHEMISTRY, 2009, 81 (14) :5821-5826

[3] Conjugated polymers as optical probes for protein interactions and protein conformations [J].

Herland, Anna ;

Inganas, Olle .

MACROMOLECULAR RAPID COMMUNICATIONS, 2007, 28 (17) :1703-1713

[4] Bio-inspired colorimetric detection of Hg2+ and Pb2+ heavy metal ions using Au nanoparticles [J].

Knecht, Marc R. ;

Sethi, Manish .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 394 (01) :33-46

[5] Biosensors for protein detection: A review [J].

Leca-Bouvier, B ;

Blum, LJ .

ANALYTICAL LETTERS, 2005, 38 (10) :1491-1517

[6] Pre-staining paper chromatography method for quantification of γ-aminobutyric acid [J].

Li, Haixing ;

Qiu, Ting ;

Cao, Yusheng ;

Yang, Jiyan ;

Huang, Zhibing .

JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (25) :5057-5060

[7] Colorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement [J].

Liang, RQ ;

Tan, CY ;

Ruan, KC .

JOURNAL OF IMMUNOLOGICAL METHODS, 2004, 285 (02) :157-163

[8] Simple telemedicine for developing regions: Camera phones and paper-based microfluidic devices for real-time, off-site diagnosis [J].

Martinez, Andres W. ;

Phillips, Scott T. ;

Carrilho, Emanuel ;

Thomas, Samuel W., III ;

Sindi, Hayat ;

Whitesides, George M. .

ANALYTICAL CHEMISTRY, 2008, 80 (10) :3699-3707

[9] Patterned paper as a platform for inexpensive, low-volume, portable bioassays [J].

Martinez, Andres W. ;

Phillips, Scott T. ;

Butte, Manish J. ;

Whitesides, George M. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (08) :1318-1320

[10] Development of rapid one-step immunochromatographic assay [J].

Paek, SH ;

Lee, SH ;

Cho, JH ;

Kim, YS .

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 2000, 22 (01) :53-60

← 1 2 →