Determination of rosiglitazone in coated tablets by MEKC and HPLC methods

Micellar electrokinetic chromatographic (MEKC) and high-performance liquid chromatographic (HPLC) methods were developed and subsequently validated for the determination of rosiglitazone (RSG) in coated tablet, a potent new oral antiltyperglicentic agent. The electrophoretic separation was performed in a fused-silica capillary of total length 48.0 cm (effective length 39.5 cm, 75 mum i.d.) using 10 mM sodium tetraborate buffer (pH 9.0) containing 30 mM sodium dodecyl sulfate (SDS) as the background electrolyte (BGE). The separating voltage used was of 20 kV at 25 degreesC and the diode array detector was set at 247 nm. The MEKC method was compared with HPLC method using a RP-18 column (125 x 4.0 mm W.) eluted with a mobile phase consisting of mixture of 25 mM potassium dihydrogen phosphate buffer and acetonitrile (55:45, v/v), adjusting the pH to 6.2 with dilute potassium hydroxide. Statistical analysis by Student's t-test showed no significant differences between the results obtained by two methods. The results indicated that MEKC can be used an alternative method to HPLC for the determination of rosiglitazone in pharmaceutical dosage form. (C) 2004 Elsevier B.V. All rights reserved.