Identification of Histoplasma capsulatum from culture extracts by real-time PCR

被引:47
作者
Martagon-Villamil, J
Shrestha, N
Sholtis, M
Isada, CM
Hall, GS
Bryne, T
Lodge, BA
Reller, LB
Procop, GW
机构
[1] Cleveland Clin Fdn, Div Infect Dis, Cleveland, OH 44195 USA
[2] Cleveland Clin Fdn, Clin Microbiol Sect, Cleveland, OH 44195 USA
[3] Duke Univ, Med Ctr, Dept Clin Microbiol, Durham, NC USA
关键词
D O I
10.1128/JCM.41.3.1295-1298.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
引用
收藏
页码:1295 / 1298
页数:4
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