Genetic characterisation of adenovirus type 8 isolated in Hiroshima city over a 15 year period

被引:26
作者
Adhikary, AK
Numaga, J
Kaburaki, T
Kawashima, H
Araie, M
Ikeda, Y
Ogino, T
Suzuki, E
Ushijima, H
Mukoyama, A
Matsuno, S
Inada, T
Okabe, N
机构
[1] Univ Tokyo, Grad Sch Med, Dept Ophthalmol, Kita Ku, Tokyo 1140024, Japan
[2] Natl Inst Infect Dis, Infect Dis Surveillance Ctr, Tokyo 1628640, Japan
[3] Hiroshima City Inst Publ Hlth, Hiroshima 7338650, Japan
[4] Univ Tokyo, Grad Sch Med, Dept Dev Med Sci, Tokyo 1130033, Japan
关键词
D O I
10.1136/jcp.56.2.120
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aims: To investigate the genetic differences among the strains of adenovirus type 8 (Ad8) circulating in Hiroshima city, Japan, and to study their circulation pattern. Methods: One hundred and twenty nine strains of adenovirus type 8 (Ad8) were isolated in Hiroshima City over a 15 year period (1983-97) from patients with keratoconjunctivitis, and analysed with six restriction enzymes-BamHI, HindIII, PstI, SacI, SalI, and Smal-to investigate possible relations among the isolates and their genetic variability. Seven hypervariable regions of the hexon gene that carry the type specific epitope were also sequenced to investigate the variation among the genome types. Results: Restriction endonuclease analyses yielded three known genome types (Ad8A, 13 samples; Ad8B, seven samples; and Ad8E, 35 samples) and a novel genome type (Ad81, 74 samples). Ad8A, Ad8B, and Ad8E were closely related, with 96% homology, whereas Ad81 had only 71% homology. Ad8A, Ad8B, and Ad8E shared 91.8% and 96.4% homology with regard to their amino acid and nucleotide sequences, respectively, with the isolate 1127 (accession no X74663). However, when compared with AAA, Ad8B, Ad8E, and isolate 1127, Ad81 shared only 62.7% and 69.9% homology with regard to amino acid and nucleotide sequences, respectively. Ad8A, Ad8B,and Ad8E had a unique 31 amino acid deletion in the hypervariable region I of the hexon gene, whereas Ad81 had a 33 residue deletion. The Ad8E strain that circulated from 1984 to 1995 was stable among the study population. Ad81 was isolated from an outbreak of epidemic keratoconjunctivitis in 1995 and was also isolated from sporadic cases until 1997. Conclusions: These results confirmed that genetic variability occurs in Ad8 in the microenvironment and revealed the emergence of a new genome type (Ad81).
引用
收藏
页码:120 / 125
页数:6
相关论文
共 22 条
  • [1] Adhikary AK, 2001, INVEST OPHTH VIS SCI, V42, P2010
  • [2] RESTRICTION SITE MAPPING OF ADENOVIRUS TYPE-8 GENOME TYPES
    ADRIAN, T
    WOLF, U
    LAUER, HJ
    WIGAND, R
    [J]. RESEARCH IN VIROLOGY, 1990, 141 (06): : 611 - 624
  • [3] Epidemic keratoconjunctivitis caused by a new genotype of adenovirus type 8 (Ad8) - A chronological review of Ad8 in southern Taiwan
    Chang, CH
    Sheu, MM
    Chern, CL
    Lin, KH
    Huang, WL
    Chen, CW
    [J]. JAPANESE JOURNAL OF OPHTHALMOLOGY, 2001, 45 (02) : 160 - 166
  • [4] DAWSON C, 1963, NEW ENGL J MED, V268, P1301
  • [5] Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively
    De Jong, JC
    Wermenbol, AG
    Verweij-Uijterwaal, MW
    Slaterus, KW
    Wertheim-Van Dillen, P
    Van Doornum, GJJ
    Khoo, SH
    Hierholzer, JC
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) : 3940 - 3945
  • [6] NEW DEVELOPMENTS IN THE MOLECULAR EPIDEMIOLOGY OF ADENOVIRUS-8 KERATOCONJUNCTIVITIS
    DEJONG, JC
    DEMAZURE, M
    LEGRANDQUILLIEN, MC
    LELAY, G
    COLIN, J
    WERMENBOL, AG
    VERWEIJUYTERWAAL, MW
    VANDERAVOORT, HGAM
    CHASTEL, C
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1992, 38 (02) : 102 - 107
  • [7] RESTRICTION ENDONUCLEASE CLEAVAGE ANALYSIS OF ADENOVIRUS TYPE-8 - 2 NEW SUBTYPES FROM PATIENTS WITH EPIDEMIC KERATOCONJUNCTIVITIS IN SAPPORO, JAPAN
    FUJII, S
    NAKAZONO, N
    SAWADA, H
    ISHII, K
    KATO, M
    AOKI, K
    OHTSUKA, H
    FUJINAGA, K
    [J]. JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1983, 36 (06): : 307 - 313
  • [8] MOLECULAR EPIDEMIOLOGY OF ADENOVIRUS TYPE-8 (AD 8) IN TAIWAN - 4 SUBTYPES RECOVERED DURING THE PERIOD OF 1980-1981 FROM PATIENTS WITH EPIDEMIC KERATOCONJUNCTIVITIS
    FUJII, S
    NAKAZONO, N
    ISHII, K
    LIN, CC
    SHEU, MM
    CHEN, CW
    FUJINAGA, K
    [J]. JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1984, 37 (04): : 161 - 169
  • [9] Construction and characterization of hexon-chimeric adenoviruses: Specification of adenovirus serotype
    Gall, JGD
    Crystal, RG
    Falck-Pedersen, E
    [J]. JOURNAL OF VIROLOGY, 1998, 72 (12) : 10260 - 10264
  • [10] COMPARATIVE-STUDIES ON ETIOLOGY AND EPIDEMIOLOGY OF VIRAL CONJUNCTIVITIS IN 3 COUNTRIES OF EAST-ASIA - JAPAN, TAIWAN AND SOUTH-KOREA
    ISHII, K
    NAKAZONO, N
    FUJINAGA, K
    FUJII, S
    KATO, M
    OHTSUKA, H
    AOKI, K
    CHEN, CW
    LIN, CC
    SHEU, MM
    LIN, KH
    OUM, BS
    LEE, SH
    CHUN, CH
    YOSHII, T
    YAMAZAKI, S
    [J]. INTERNATIONAL JOURNAL OF EPIDEMIOLOGY, 1987, 16 (01) : 98 - 103