Characterization of expanded T cell clones in healthy macaques:: ontogeny, distribution and stability

被引:5
作者
Nam, KH
Illés, Z
Terao, K
Yoshikawa, Y
Yamamura, T
机构
[1] Natl Inst Infect Dis, Tsukuba Primate Ctr, Tsukuba, Ibaraki 3050843, Japan
[2] Univ Tokyo, Fac Agr, Dept Biomed Sci, Bunkyo Ku, Tokyo 1138657, Japan
[3] NCNP, Natl Inst Neurosci, Dept Demyelinating Dis & Aging, Tokyo 1870031, Japan
关键词
cymolgus monkey; peripheral lymphocyte; T cell; clonal expansion; TCR beta chain; aging; RT-PCRI; SSCP;
D O I
10.1016/S0145-305X(00)00004-5
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Peripheral expanded T cell clones have been discussed mainly in relation to certain diseases or immune function in humans and mice. There is little information on their ontogeny, stability and distribution among T cell subsets as well as major lymphoid organs, We applied reverse transcription-polymerase chain reaction (RT-PCR) with family specific primers for monkey T cell receptor beta chain V regions and single-strand conformation polymorphism (SSCP) analysis to analyze the expanded T cell clones in cynomolgus monkeys (Macaca fascicularis). A number of expanded T cell clones were detected in the peripheral blood of young and adult monkeys, but few expanded T cell clones were detected in the blood of a fetus and a 2-day-old neonate. The clones in adults were maintained over 3 months. These expanded T cell clones were distributed only in peripheral blood and spleen, but few were found in lymph nodes (axillary, inguinal and intestinal). The number of expanded T cell clones was much greater in CD8 single-positive (CD8sp) T cells than in CD4sp T cells, showing that most of these clones originated in the CD8sp T cell population. Almost all the expanded CD8sp T cell clones belonged to the CD28(-), CD29(hi) and Fas(+) subset. The usage of V beta genes was not skewed in the 24 V beta. Furthermore, higher mRNA signals for effector molecules perforin and IFN-gamma were detected in CD8sp T cell subsets with phenotypes of CD28-, CD29(hi) and Fas(+), suggesting that the expanded T cells might have developed in relation to T cell activation in the periphery of cynomolgus monkeys. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:703 / 715
页数:13
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