Quantitative detection of protein expression in single cells using droplet microfluidics

被引：305

作者：

Huebner, A.

Srisa-Art, M.

Holt, D.

Abell, C.

Hollfelder, F.

deMello, A. J.

Edel, J. B.

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[3] Univ London Imperial Coll Sci Technol & Med, Dept Chem, Kensington SW7 2AZ, England

[4] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, Kensington SW7 2AZ, England

来源：

CHEMICAL COMMUNICATIONS | 2007年 / 12期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1039/b618570c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.

引用

页码：1218 / 1220

页数：3

共 23 条

[1] High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments
Aharoni, A
Amitai, G
Bernath, K
Magdassi, S
Tawfik, DS
[J]. CHEMISTRY & BIOLOGY, 2005, 12 (12): : 1281 - 1289
[2] In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting
Bernath, K
Hai, MT
Mastrobattista, E
Griffiths, AD
Magdassi, S
Tawfik, DS
[J]. ANALYTICAL BIOCHEMISTRY, 2004, 325 (01) : 151 - 157
[3] Microfluidics-based systems biology
Breslauer, DN
Lee, PJ
Lee, LP
[J]. MOLECULAR BIOSYSTEMS, 2006, 2 (02) : 97 - 112
[4] Microfluidic systems for chemical kinetics that rely on chaotic mixing in droplets
Bringer, MR
Gerdts, CJ
Song, H
Tice, JD
Ismagilov, RF
[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2004, 362 (1818): : 1087 - 1104
[5] Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening
Chen, Delai L.
Ismagilov, Rustem F.
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2006, 10 (03) : 226 - 231
[6] Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization
Cohen, HM
Tawfik, DS
Griffiths, AD
[J]. PROTEIN ENGINEERING DESIGN & SELECTION, 2004, 17 (01) : 3 - 11
[7] Flow cytometric screening of cell-based libraries
Daugherty, PS
Iverson, BL
Georgiou, G
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 243 (1-2) : 211 - 227
[8] DNA fragment sizing by single molecule detection in submicrometer-sized closed fluidic channels
Foquet, M
Korlach, J
Zipfel, W
Webb, WW
Craighead, HG
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (06) : 1415 - 1422
[9] Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization
Griffiths, AD
Tawfik, DS
[J]. EMBO JOURNAL, 2003, 22 (01) : 24 - 35
[10] Miniaturising the laboratory in emulsion droplets
Griffiths, Andrew D.
Tawfik, Dan S.
[J]. TRENDS IN BIOTECHNOLOGY, 2006, 24 (09) : 395 - 402

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