Preparation of a monoclonal antibody to Nε-(hexanonyl)lysine:: Application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle

被引：53

作者：

Kato, Y ^{[1
]}

Miyake, Y ^{[1
]}

Yamamoto, K ^{[1
]}

Shimomura, Y ^{[1
]}

Ochi, H ^{[1
]}

Mori, Y ^{[1
]}

Osawa, T ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 274卷 / 02期

关键词：

monoclonal antibody; lipid hydroperoxide; exercise; oxidative stress; flavonoid; biomarker; adduct;

D O I：

10.1006/bbrc.2000.3150

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The monoclonal antibody to N-epsilon-(hexanonyl) lysine (HEL), a novel adduct formed by the reaction of linoleic acid hydroperoxide and lysine, has been prepared and characterized. The obtained antibody specifically recognized the HEL moiety, Using the monoclonal antibody, we evaluated the protective effects of feeding eriocitrin, which is one of flavonoids in lemon fruit, on oxidative modification induced by exercise in rats. The supplementation of eriocitrin significantly suppressed the increase in HEL in the skeletal muscle by exercise. The result suggests that the determination of HEL may be a good method for evaluation of the protective effect of beneficial food factors against oxidative stress. (C) 2000 Academic Press.

引用

页码：389 / 393

页数：5

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