β-1,3 glucan as a test for central venous catheter biofilm infection

被引:61
作者
Nett, Jeniel
Lincoln, Leslie
Marchillo, Karen
Andes, David
机构
[1] Univ Wisconsin, Dept Med, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
关键词
D O I
10.1086/517522
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Biofilms are microbial communities that are associated with solid surfaces such as intravascular catheters. Candida species are a major cause of medical device-associated infections. Twenty percent to 70% of all candidemias are associated with this biofilm process. Diagnosis and effective treatment of Candida deviceassociated infections requires removal of the involved device. The ability to identify a biofilm device infection before catheter removal may obviate removal of a substantial number of devices. Prior studies in our laboratory identified cell wall changes (specifically, increased beta-1,3 glucan) associated with biofilm, compared with planktonic C. albicans. Both in vitro and in vivo (catheter) biofilm models were used to determine whether biofilm cells secreted more b- 1,3 glucan and whether these differences could be used to discern the presence of a Candida biofilm infection with 3 species (C. albicans, C. glabrata, and C. parapsilosis). A limulus lysate assay was used to quantify b- 1,3 glucan in supernatants from planktonic or biofilm cultures and in the serum of rats with an intravascular catheter biofilm infection or disseminated candidiasis. b- 1,3 glucan was detected from both in vitro and in vivo models from each condition. However, the concentrations of b- 1,3 glucan from the biofilm conditions were 4-10-fold greater in vitro (P < .001) and were 10- fold greater in vivo (P < .001) despite equal or fewer numbers of cells in the biofilm conditions. These results suggest the secreted polysaccharide beta-1,3 glucan may serve as a useful tool for the diagnosis of Candida biofilm and device- associated infections.
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页码:1705 / 1712
页数:8
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