Detection of human cytomegalovirus DNA by real-time quantitative PCR

被引:45
作者
Nitsche, A
Steuer, N
Schmidt, CA
Landt, O
Ellerbrok, H
Pauli, G
Siegert, W
机构
[1] Humboldt Univ, Klin Innere Med MS Hamatol & Onkol, D-13353 Berlin, Germany
[2] Robert Koch Inst, TIB Molbiol, D-1000 Berlin, Germany
[3] Robert Koch Inst, Dept Virol, D-1000 Berlin, Germany
关键词
D O I
10.1128/JCM.38.7.2734-2737.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.
引用
收藏
页码:2734 / 2737
页数:4
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