Differences in the second internal transcribed spacer of four species of Nematodirus (Nematoda: Molineidae)

被引：39

作者：

Newton, LA ^{[1
]}

Chilton, NB ^{[1
]}

Beveridge, I ^{[1
]}

Gasser, RB ^{[1
]}

机构：

[1] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1998年 / 28卷 / 02期

基金：

澳大利亚研究理事会;

关键词：

rDNA; ITS-2; Nematodirus spathiger; Nematodirus filicollis; Nematodirus helvetianus; Nematodirus battus;

D O I：

10.1016/S0020-7519(97)00150-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Genetic differences among Nematodirus spathiger, Nematodirus filicollis, Nematodirus helvetianus and Nematodirus battus in the nucleotide sequence of the second internal transcribed spacer (ITS-2) of ribosomal DNA ranged from 3.9 to 24.7%. Pairwise comparisons of their ITS-2 sequences indicated that the most genetically similar species were N. spathiger and N. helvetianus. N. battus was the most genetically distinct species, with differences ranging from 22.8 to 24.7% with respect to the other three species. Some of the nucleotide differences among species provided different endonuclease restriction sites that could be used in restriction fragment length polymorphism studies, The ITS-2 sequence data may prove useful in studies of the systematics of molineid nematodes. (C) 1998 Australian Society for Parasitology. Published by Elsevier Science Ltd.

引用

页码：337 / 341

页数：5

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