A method for fluorescence in situ hybridization against synaptonemal complex-associated chromatin of plant meiocytes

被引:10
作者
Cuñado, N [1 ]
Santos, JL [1 ]
机构
[1] Univ Complutense Madrid, Fac Biol, Dept Genet, E-28040 Madrid, Spain
关键词
D O I
10.1006/excr.1997.3892
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
An improved method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorencent in situ hybridization is described. This technique produces clear preparations of SCs and, in addition, consistently reveals the organization and location of different repetitive DNA sequences in plant meiotic prophase chromosomes. (C) 1998 Academic Press.
引用
收藏
页码:179 / 182
页数:4
相关论文
共 25 条
[1]   INSITU LOCALIZATION OF 2 REPETITIVE DNA-SEQUENCES TO SURFACE-SPREAD PACHYTENE CHROMOSOMES OF RYE [J].
ALBINI, SM ;
SCHWARZACHER, T .
GENOME, 1992, 35 (04) :551-559
[2]   XY chromosome behaviour in the germ-line of the human male: a FISH analysis of spatial orientation, chromatin condensation and pairing [J].
Armstrong, Susan J. ;
Kirkham, Amanda J. ;
Hulten, Maj A. .
CHROMOSOME RESEARCH, 1994, 2 (06) :445-452
[3]   Genome differentiation in Aegilops .2. Physical mapping of 5S and 18S-26S ribosomal RNA gene families in diploid species [J].
Badaeva, ED ;
Friebe, B ;
Gill, BS .
GENOME, 1996, 39 (06) :1150-1158
[4]   Electron microscopic in situ hybridization (EMISH) against synaptonemal complex-associated chromatin [J].
Barlow, AL .
CHROMOSOME RESEARCH, 1996, 4 (03) :248-249
[5]   Combined immunocytogenetic and molecular cytogenetic analysis of meiosis I human spermatocytes [J].
Barlow, AL ;
Hulten, MA .
CHROMOSOME RESEARCH, 1996, 4 (08) :562-573
[6]   MOLECULAR DESCRIPTION OF TELOMERIC HETEROCHROMATIN IN SECALE SPECIES [J].
BEDBROOK, JR ;
JONES, J ;
ODELL, M ;
THOMPSON, RD ;
FLAVELL, RB .
CELL, 1980, 19 (02) :545-560
[7]   COMPARISON OF PLANT TELOMERE LOCATIONS USING A PCR-GENERATED SYNTHETIC PROBE [J].
COX, AV ;
BENNETT, ST ;
PAROKONNY, AS ;
KENTON, A ;
CALLIMASSIA, MA ;
BENNETT, MD .
ANNALS OF BOTANY, 1993, 72 (03) :239-247
[8]   Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization [J].
Cuadrado, A ;
Ceoloni, C ;
Jouve, N .
GENOME, 1995, 38 (06) :1061-1069
[9]  
FERNANDEZCALVIN B, 1995, CHROMOSOMA, V103, P554, DOI 10.1007/BF00355320
[10]   CLONING AND CHARACTERIZATION OF RIBOSOMAL-RNA GENES FROM WHEAT AND BARLEY [J].
GERLACH, WL ;
BEDBROOK, JR .
NUCLEIC ACIDS RESEARCH, 1979, 7 (07) :1869-1885