Connexin 43 expression promotes malignancy of HuH7 hepatocellular carcinoma cells via the inhibition of cell-cell communication

被引：41

作者：

Zhang, Dan

Kaneda, Makoto

Nakahama, Ken-ichi

Arii, Shigeki

Morita, Ikuo

机构：

[1] Tokyo Med & Dent Univ, Dept Cell Physiol Chem, Bunkyo Ku, Tokyo, Japan

[2] Tokyo Med & Dent Univ, Grad Sch, Dept Hepatobiliary Pancreat Surg, Bunkyo Ku, Tokyo, Japan

来源：

CANCER LETTERS | 2007年 / 252卷 / 02期

基金：

日本学术振兴会;

关键词：

Connexin; 43; 32; gap junction; gap junctional intercellular communication; hepatocellular carcinoma;

D O I：

10.1016/j.canlet.2006.12.024

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In normal liver, Connexin (Cx) 43 is not detected, but up-regulated in some liver cancers. We herein investigated the role of Cx43 in hepatoma cell carcinogenesis. Cx43-silenced HuH7 cells using shRNA showed lower growth and higher differentiation, and Cx43-overexpressing cells exhibited rapid growth and low differentiation. Unexpectedly, gap junctional intercellular communication (GJIC) was inversely correlated with Cx43 levels. Furthermore, the expression level and promoter activity of Cx32 was negatively regulated by the expression of Cx43. From these data, Cx43 expression may be in part responsible for the malignancy of hepatoma cells through a decrease in GJIC composed of Cx32. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：208 / 215

页数：8

共 28 条

[1] Human Connexin 32, a gap junction protein altered in the X-linked form of Charcot-Marie-Tooth disease, is directly regulated by the transcription factor SOX10
Bondurand, N
Girard, M
Pingault, V
Lemort, N
Dubourg, O
Goossens, M
[J]. HUMAN MOLECULAR GENETICS, 2001, 10 (24) : 2783 - 2795
[2] Identification of functional regulatory regions of the connexin32 gene promoter
Field, JML
Tate, LA
Chipman, JK
Minchin, SD
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2003, 1628 (01): : 22 - 29
[3] Opposite regulation of connexin33 and connexin43 by LPS and IL-1α in spermatogenesis
Fiorini, C
Decrouy, X
Defamie, N
Segretain, D
Pointis, G
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2006, 290 (03): : C733 - C740
[4] Is the junctional uncoupling elicited in rat ventricular myocytes by some dephosphorylation treatments due to changes in the phosphorylation status of Cx43?
Hervé, JC
Plaisance, I
Loncarek, J
Duthe, F
Sarrouilhe, D
[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2004, 33 (03): : 201 - 210
[5] Huang RC, 2002, CANCER RES, V62, P2806
[6] Increased expression of connexin 26 in the invasive component of lung squamous cell carcinoma: Significant correlation with poor prognosis
Ito, A
Koma, Y
Uchino, K
Okada, T
Ohbayashi, C
Tsubota, N
Okada, M
[J]. CANCER LETTERS, 2006, 234 (02) : 239 - 248
[7] Connexins as targets for cancer chemoprevention and chemotherapy
King, TJ
Bertram, JS
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2005, 1719 (1-2): : 146 - 160
[8] Mice deficient for the gap junction protein Connexin32 exhibit increased radiation-induced tumorigenesis associated with elevated mitogen-activated protein kinase (p44/Erk1, p42/Erk2) activation
King, TJ
Lampe, PD
[J]. CARCINOGENESIS, 2004, 25 (05) : 669 - 680
[9] Influence of transfection with connexin 26 gene on malignant potential of human hepatoma cells
Muramatsu, A
Iwai, M
Morikawa, T
Tanaka, S
Mori, T
Harada, Y
Okanoue, T
[J]. CARCINOGENESIS, 2002, 23 (02) : 351 - 358
[10] Expression of gap junction protein connexin32 in chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma
Nakashima, Y
Ono, T
Yamanoi, A
El-Assal, ON
Kohno, H
Nagasue, N
[J]. JOURNAL OF GASTROENTEROLOGY, 2004, 39 (08) : 763 - 768

← 1 2 3 →