Individual molecules of dye-labeled DNA act as a reversible two-color switch upon application of an electric field

被引：8

作者：

White, SS ^{[1
]}

Ying, LM ^{[1
]}

Balasubramanian, S ^{[1
]}

Klenerman, D ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 44期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DNA; FRET (fluorescence resonance energy transfer) molecular devices; nanotechnology; single-molecule studies;

D O I：

10.1002/anie.200460323

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Reversible switching of the fluorescence of an individual dye-labeled DNA molecule is possible by utilizing an electric field applied in the tip of a nanopipette (see picture; FRET = fluorescence resonance energy transfer). The electric field appears to alter the conformation of the acceptor dye only, resulting in a significant change in its quantum yield.

引用

收藏

页码：5926 / 5930

页数：5

相关论文

共 35 条

[1] DNA duplex-quadruplex exchange as the basis for a nanomolecular machine [J].

Alberti, P ;

Mergny, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (04) :1569-1573

[2] DNA molecule provides a computing machine with both data and fuel [J].

Benenson, Y ;

Adar, R ;

Paz-Elizur, T ;

Livneh, Z ;

Shapiro, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2191-2196

[3] Influencing intramolecular motion with an alternating electric field [J].

Bermudez, V ;

Capron, N ;

Gase, T ;

Gatti, FG ;

Kajzar, F ;

Leigh, DA ;

Zerbetto, F ;

Zhang, SW .

NATURE, 2000, 406 (6796) :608-611

[4] Excitonic heterodimer formation in an HIV-1 oligonucleotide labeled with a donor-acceptor pair used for fluorescence resonance energy transfer [J].

Bernacchi, S ;

Piémont, E ;

Potier, N ;

van Dorsselaer, A ;

Mély, Y .

BIOPHYSICAL JOURNAL, 2003, 84 (01) :643-654

[5]

Bernacchi S, 2001, Nucleic Acids Res, V29, pE62, DOI 10.1093/nar/29.13.e62

[6] A CHEMICALLY AND ELECTROCHEMICALLY SWITCHABLE MOLECULAR SHUTTLE [J].

BISSELL, RA ;

CORDOVA, E ;

KAIFER, AE ;

STODDART, JF .

NATURE, 1994, 369 (6476) :133-137

[7] DNA-templated assembly and electrode attachment of a conducting silver wire [J].

Braun, E ;

Eichen, Y ;

Sivan, U ;

Ben-Yoseph, G .

NATURE, 1998, 391 (6669) :775-778

[8] Writing with DNA and protein using a nanopipet for controlled delivery [J].

Bruckbauer, A ;

Ying, LM ;

Rothery, AM ;

Zhou, DJ ;

Shevchuk, AI ;

Abell, C ;

Korchev, YE ;

Klenerman, D .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (30) :8810-8811

[9] SYNTHESIS FROM DNA OF A MOLECULE WITH THE CONNECTIVITY OF A CUBE [J].

CHEN, JH ;

SEEMAN, NC .

NATURE, 1991, 350 (6319) :631-633

[10] Single-pair fluorescence resonance energy transfer on freely diffusing molecules: Observation of Forster distance dependence and subpopulations [J].

Deniz, AA ;

Dahan, M ;

Grunwell, JR ;

Ha, TJ ;

Faulhaber, AE ;

Chemla, DS ;

Weiss, S ;

Schultz, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :3670-3675

← 1 2 3 4 →