Effect of fixation period on HER2/neu gene amplification detected by fluorescence in situ hybridization in invasive breast carcinoma

被引：37

作者：

Selvarajan, S

Bay, BH

Choo, A

Chuah, KL

Sivaswaren, CR

Tien, SL

Wong, CY

Tan, PH

机构：

[1] Singapore Gen Hosp, Dept Pathol, Singapore 169608, Singapore

[2] Singapore Gen Hosp, Dept Gen Surg, Singapore 169608, Singapore

[3] Natl Univ Singapore, Dept Anat, Singapore 117548, Singapore

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2002年 / 50卷 / 12期

关键词：

FISH; HER2/neu; fixation; breast cancer; immunohistochemistry;

D O I：

10.1177/002215540205001215

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study investigated if formalin fixation duration affects HER2/neu gene amplification detection by fluorescence in situ hybridization (FISH) in breast cancer. Tumor tissues from 35 cases were divided into three groups and subjected to two formalin fixation protocols per group (12 hr, 27 hr in the first; 2 hr, 17.5 hr in the second; 28.5 hr, 541 hr in the third) before FISH analysis. There was no significant difference in FISH signal detection between the two different fixation protocols in the first two groups. In the third, no signal was detected in 4/6 cases fixed for an extended duration.

引用

页码：1693 / 1696

页数：4

共 12 条

[1] Strong correlation between results of fluorescent in situ hybridization and immunohistochemistry for the assessment of the ERBB2 (HER-2/neu) gene status in breast carcinoma [J].