The Potential of Human Allogeneic Juvenile Chondrocytes for Restoration of Articular Cartilage

被引:191
作者
Adkisson, H. Davis [1 ]
Martin, James A. [2 ]
Amendola, Richard L. [2 ]
Milliman, Curt [1 ]
Mauch, Kelsey A. [4 ]
Katwal, Arbindra B. [1 ]
Seyedin, Mitchell [1 ]
Amendola, Annuziato [2 ]
Streeter, Philip R. [4 ]
Buckwalter, Joseph A. [2 ,3 ]
机构
[1] ISTO Technol Inc, St Louis, MO USA
[2] Univ Iowa Orthopaed & Rehabil, Iowa City, IA USA
[3] Iowa City Vet Adm Med Ctr, Iowa City, IA USA
[4] OHSU Knight Canc Inst, Ctr Hematol Malignancies, Oregon Stem Cell Ctr, Portland, OR USA
基金
美国国家卫生研究院;
关键词
juvenile human chondrocytes; neocartilage; agarose culture; immunogenicity; serial expansion; cartilage repair; chondrocyte transplantation; aging; COLLAGEN-IX; OSTEOCHONDRAL ALLOGRAFTS; EXTRACELLULAR-MATRIX; RANDOMIZED-TRIAL; KNEE; TRANSPLANTATION; IMPLANTATION; REPAIR; MICROFRACTURE; JOINT;
D O I
10.1177/0363546510361950
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
100224 [整形外科学];
摘要
Background: Donor-site morbidity, limited numbers of cells, loss of phenotype during ex vivo expansion, and age-related decline in chondrogenic activity present critical obstacles to the use of autologous chondrocyte implantation for cartilage repair. Chondrocytes from juvenile cadaveric donors may represent an alternative to autologous cells. Hypothesis/Purpose: The authors hypothesized that juvenile chondrocyte would show stronger and more stable chondrogenic activity than adult cells in vitro and that juvenile cells pose little risk of immunologic incompatibility in adult hosts. Study Design: Controlled laboratory study. Methods: Cartilage samples were from juvenile (<13 years old) and adult (>13 years old) donors. The chondrogenic activity of freshly isolated human articular chondrocytes and of expanded cells after monolayer culture was measured by proteoglycan assay, gene expression analysis, and histology. Lymphocyte proliferation assays were used to assess immunogenic activity. Results: Proteoglycan content in neocartilage produced by juvenile chondrocytes was 100-fold higher than in neocartilage produced by adult cells. Collagen type II and type IX mRNA in fresh juvenile chondrocytes were 100- and 700-fold higher, respectively, than in adult chondrocytes. The distributions of collagens II and IX were similar in native juvenile cartilage and in neocartilage made by juvenile cells. Juvenile cells grew significantly faster in monolayer cultures than adult cells (P=.002) and proteoglycan levels produced in agarose culture was significantly higher in juvenile cells than in adult cells after multiple passages (P<.001). Juvenile chondrocytes did not stimulate lymphocyte proliferation. Conclusion: These results document a dramatic age-related decline in human chondrocyte chondrogenic potential and show that allogeneic juvenile chondrocytes do not stimulate an immunologic response in vivo. Clinical Relevance: Juvenile human chondrocytes have greater potential to restore articular cartilage than adult cells, and may be transplanted without the fear of rejection, suggesting a new allogeneic approach to restoring articular cartilage in older individuals.
引用
收藏
页码:1324 / 1333
页数:10
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