Ferrocene branched chitosan for the construction of a reagentless amperometric hydrogen peroxide biosensor

被引：46

作者：

Garcia, Ariel

Peniche-Covas, Carlos

Chico, Belkis

Simpson, Benjamin K.

Villalonga, Reynaldo

机构：

[1] Univ Matanzas, Ctr Enzyme Technol, Matanzas 44740, Cuba

[2] Univ Havana, Ctr Biomat, Havana 10400, Cuba

[3] McGill Univ, Food Sci & Agr Chem Dept, Ste Anne De Bellevue, PQ H9X 3V9, Canada

来源：

MACROMOLECULAR BIOSCIENCE | 2007年 / 7卷 / 04期

关键词：

biosensors; chitosan; immobilized enzymes; polysaccharides;

D O I：

10.1002/mabi.200700032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitosan was chemically branched with ferrocene moieties and further used as a support for the immobilization of horseradish peroxidase on a glassy carbon electrode. The reagentless biosensor device showed a linear amperometric response toward hydrogen peroxide concentrations between 35 X 10(-6) M and 2.0 x 10(-3) M. The biosensor reached 95% of the steady-state current in about 20 s and its sensitivity was 28.4 x 10(-3) mu A.M-1. The enzyme electrode retained 94% of its initial activity after 2 weeks of storage at 4 degrees C in 50 x 10(-3) M sodium phosphate buffer at pH 7.0.

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收藏

页码：435 / 439

页数：5

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