Immunogenicity of a subunit vaccine against Bacillus anthracis

被引：61

作者：

Chichester, Jessica A. ^{[1
]}

Musiychuk, Konstantin ^{[1
]}

de la Rosa, Patricia ^{[1
]}

Horsey, April ^{[1
]}

Stevenson, Natalie ^{[1
]}

Ugulava, Natalia ^{[1
]}

Rabindran, Shailaja ^{[1
]}

Palmer, Gene A. ^{[1
]}

Mett, Vadim ^{[1
]}

Yusibov, Vidadi ^{[1
]}

机构：

[1] Fraunhofer USA Ctr Mol Biotechnol, Newark, DE 19711 USA

来源：

VACCINE | 2007年 / 25卷 / 16期

关键词：

anthrax; subunit vaccine; plant expression;

D O I：

10.1016/j.vaccine.2007.01.068

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The current approved vaccine against anthrax is based on protective antigen (PA) of Bacillus anthracis, requires six injections over an 18-month period and has a known history of side effects. Therefore, there is significant effort towards developing an improved vaccine against B. anthracis. Here we separately engineered and expressed domain 4 of PA (PAD4) and domain I of lethal factor (LFD1) as fusions to lichenase (LicKM), a thermostable enzyme from Clostridium thermocellum, and transiently expressed these fusions in Nicotiana benthamiana. Plant-produced antigens were combined and immunogenicity was evaluated in mice. All animals that received the experimental vaccine developed high antibody titers that were predominantly IgGI and were able to neutralize the effects of LeTx in vitro. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：3111 / 3114

页数：4

共 13 条

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