Mortality of eggs and third instar larvae of Anastrepha ludens and A-obliqua with insecticidal controlled atmospheres at high temperatures

The in vitro mortality of eggs and third instar larvae of Anastrepha ludens and A. obliqua was determined after exposure to 21 treatments of air or controlled atmospheres (CA) at high temperatures and 50% RH. Air at 44 degreesC for 160 min caused very low mortality, which increased significantly by CA. Higher temperatures caused a more rapid kill. One hundred percent mortality was achieved for third instar larvae of both species in air or CA at 48 degreesC for 220 min. A 100% mortality of eggs of A. ludens was achieved in air at 51 degreesC for 240 min or in CA at 52 degreesC for 240 min, and 100% mortality of eggs of A. obliqua was achieved in air or in CA at 55 degreesC for 240 min. A. obliqua was slightly more tolerant than A. ludens, and eggs were more tolerant than third instar larvae in both species. CA had a synergistic effect at <50<degrees>C, but was slightly less effective than air at higher temperatures. Low O-2 concentrations were more effective than high CO2 levels. The mean estimated temperatures for 50, 99 and 99.9968% mortality (LT(50)s, LT(99)s, LT(99.9968)s) of eggs of A. obliqua (the most tolerant) exposed to 0 kPa O-2 + 50 kPa CO2 for 240 min were 49.4, 54.8 and 60.9 degreesC, respectively. We conclude that dry hot air at greater than or equal to 44 degreesC and 50% RH in CA (0 kPa O-2 + 50 kPa CO2), for 160 min or longer, is effective in increasing mortality of eggs and third instar larvae of A. ludens and A. obliqua. (C) 2000 Elsevier Science B.V. All rights reserved.