Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method

被引：85

作者：

Song, TY ^{[1
]}

Toma, C ^{[1
]}

Nakasone, N ^{[1
]}

Iwanaga, M ^{[1
]}

机构：

[1] Univ Ryukyus, Grad Sch Med, Dept Microbiol, Div Bacterial Pathogenesis, Nishihara, Okinawa 9030215, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 243卷 / 01期

关键词：

Shigella; enteroinvasive; Escherichia coli; LAMP; ipaH gene detection;

D O I：

10.1016/j.femsle.2004.12.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

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页码：259 / 263

页数：5

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