Fluorescent timer: Protein that changes color with time

被引：315

作者：

Terskikh, A ^{[1
]}

Fradkov, A

Ermakova, G

Zaraisky, A

Tan, P

Kajava, AV

Zhao, XN

Lukyanov, S

Matz, M

Kim, S

Weissman, I

Siebert, P

机构：

[1] Stanford Univ, Sch Med, Stanford, CA 94305 USA

[2] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117871, Russia

[3] NIH, Ctr Mol Modeling, Ctr Informat Technol, Bethesda, MD 20892 USA

[4] Clontech Labs, Palo Alto, CA 94303 USA

来源：

SCIENCE | 2000年 / 290卷 / 5496期

关键词：

D O I：

10.1126/science.290.5496.1585

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We generated a mutant of the red fluorescent protein drFP583. The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression. We used in vivo labeling with E5 to measure expression from the heat shock-dependent promoter in Caenorhabditis elegans and from the Otx-2 promoter in developing Xenopus embryos. Thus, E5 is a "fluorescent timer" that can be used to monitor both activation and downregulation of target promoters on the whole-organism scale.

引用

页码：1585 / 1588

页数：4

共 16 条

[1]

Cadwell R C, 1992, PCR Methods Appl, V2, P28, DOI 10.1101/gr.2.1.28

[2] RED-SHIFTED EXCITATION MUTANTS OF THE GREEN FLUORESCENT PROTEIN [J].

DELAGRAVE, S ;

HAWTIN, RE ;

SILVA, CM ;

YANG, MM ;

YOUVAN, DC .

BIO-TECHNOLOGY, 1995, 13 (02) :151-154

[3] GREEN-FLUORESCENT PROTEIN MUTANTS WITH ALTERED FLUORESCENCE EXCITATION-SPECTRA [J].

EHRIG, T ;

OKANE, DJ ;

PRENDERGAST, FG .

FEBS LETTERS, 1995, 367 (02) :163-166

[4]

Ermakova GV, 1999, DEVELOPMENT, V126, P4513

[5]

ETKIN LD, 1987, DEVELOPMENT, V99, P15

[6] Origin of anterior patterning - how old is our head? [J].

Galliot, B ;

Miller, D .

TRENDS IN GENETICS, 2000, 16 (01) :1-5

[7]

HELM R, 1994, P NATL ACAD SCI USA, V91, P12501

[8]

Helm R., 1995, Nature, V373, P663

[9] DIFFERENTIAL REGULATION OF CLOSELY RELATED MEMBERS OF THE HSP16 GENE FAMILY IN CAENORHABDITIS-ELEGANS [J].

JONES, D ;

DIXON, DK ;

GRAHAM, RW ;

CANDIDO, EPM .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1989, 8 (07) :481-490

[10] Fluorescent proteins from nonbioluminescent Anthozoa species [J].

Matz, MV ;

Fradkov, AF ;

Labas, YA ;

Savitsky, AP ;

Zaraisky, AG ;

Markelov, ML ;

Lukyanov, SA .

NATURE BIOTECHNOLOGY, 1999, 17 (10) :969-973

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