Two genes of the anaerobic fungus Orpinomyces sp. strain PC-2 encoding cellulases with endoglucanase activities may have arisen by gene duplication

被引：17

作者：

Chen, HZ

Li, XL

Blum, DL

Ljungdahl, LG ^{[1
]}

机构：

[1] Univ Georgia, Ctr Biol Resource Recovery, Athens, GA 30602 USA

[2] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 159卷 / 01期

关键词：

endoglucanase; Orpinomyces PC-2; gene duplication; celE;

D O I：

10.1016/S0378-1097(97)00546-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cDNA designated celE cloned from Orpinomyces PC-2 consisted of an open reading frame encoding a polypeptide (CelE) of 477 amino acids. CelE was highly homologous to CelBs of Orpinomyces (72.3% identity) and Neocallimastix (67.9% identity) and like them it had a non-catalytic repeated peptide domain (NCRPD) at the C-terminal end. The catalytic domain of CelE was homologous to glycosyl hydrolases of Family 5, found in several anaerobic bacteria. The gene of celE was devoid of introns. The recombinant proteins CelE and CelB of Orpinomyces PC-2 randomly hydrolyzed carboxymethylcellulose and cello-oligosaccharides in the pattern of endoglucanases. The results indicated that a gene of bacterial origin was duplicated to form celE and celB of Orpinomyces PC-2. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

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页码：63 / 68

页数：6

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