Binding characteristics of CebR, the regulator of the ceb operon required for cellobiose/cellotriose uptake in Streptomyces reticuli

被引：10

作者：

Schlösser, A ^{[1
]}

Aldekamp, T ^{[1
]}

Schrempf, H ^{[1
]}

机构：

[1] Univ Osnabruck, Fachbereich Biol Chem, D-49069 Osnabruck, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 190卷 / 01期

关键词：

cellobiose/cellatriose ABC transporter; CebR regulator; streptomycete; actinomycete;

D O I：

10.1016/S0378-1097(00)00304-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Streptomyces reticuli Avicelase (cellulase, Cell) hydrolyzes crystalline cellulose to cellooligomers, cellobiose and cellotriose which are taken up by mycelia via an ABC transport system (Ceb) induced during growth with cellobiose or cellulose. The cebR gene located upstream of the cebEFG operon was cloned in Escherichia coli in frame with six histidine-encoding codons. The resulting purified fusion protein was shown to bind to a motif of 23 bp, including a perfect 18-bp palindrome situated upstream of the cebEFG. Cytoplasmic extracts of induced, but not of uninduced S. reticuli protected the same DNA motif. Release of the CebR regulator from its operator occurs upon addition of cellopentaose which can be assumed to act as inducer within the mycelia. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：127 / 132

页数：6

共 16 条

[1]

Ausubel FM, 1995, SHORT PROTOCOLS MOL

[2] TRANSCRIPTION REGULATION BY INFLEXIBILITY OF PROMOTER DNA IN A LOOPED COMPLEX [J].

CHOY, HE ;

PARK, SW ;

PARRACK, P ;

ADHYA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7327-7331

[3] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[4] Crystal structure of the lactose operon repressor and its complexes with DNA and inducer [J].

Lewis, M ;

Chang, G ;

Horton, NC ;

Kercher, MA ;

Pace, HC ;

Schumacher, MA ;

Brennan, RG ;

Lu, PZ .

SCIENCE, 1996, 271 (5253) :1247-1254

[5]

Sambrook J., 2002, MOL CLONING LAB MANU

[6] BIOCHEMICAL AND ELECTRON-MICROSCOPIC STUDIES OF THE STREPTOMYCES-RETICULI CELLULASE (AVICELASE) IN ITS MYCELIUM-ASSOCIATED AND EXTRACELLULAR FORMS [J].

SCHLOCHTERMEIER, A ;

NIEMEYER, F ;

SCHREMPF, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (10) :3240-3248

[7] THE GENE ENCODING THE CELLULASE (AVICELASE) CEL1 FROM STREPTOMYCES-RETICULI AND ANALYSIS OF PROTEIN DOMAINS [J].

SCHLOCHTERMEIER, A ;

WALTER, S ;

SCHRODER, J ;

MOORMAN, M ;

SCHREMPF, H .

MOLECULAR MICROBIOLOGY, 1992, 6 (23) :3611-3621

[8]

Schlösser A, 1999, APPL ENVIRON MICROB, V65, P2636

[9] A lipid-anchored binding protein is a component of an ATP-dependent cellobiose/cellotriose-transport system from the cellulose degrader Streptomyces reticuli [J].

Schlosser, A ;

Schrempf, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 242 (02) :332-338

[10] The Streptomyces ATP-binding component MsiK assists in cellobiose and maltose transport [J].

Schlosser, A ;

Kampers, T ;

Schrempf, H .

JOURNAL OF BACTERIOLOGY, 1997, 179 (06) :2092-2095

← 1 2 →