Continuous penicillin G hydrolysis in an electro-membrane reactor with immobilized penicillin G acylase

被引：10

作者：

Zadrazil, A

Chmelíková, R

Hasal, P

机构：

[1] Inst Chem Technol, CR-16628 Prague 6, Czech Republic

[2] Ctr Nonlinear Dynam Chem & Biol Syst, Prague 16628 6, Czech Republic

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 06期

关键词：

electric field; immobilized enzyme; mass transfer; membrane reactor; penicillin acylase;

D O I：

10.1023/A:1022656129744

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Penicillin G (2%, w/v in phosphate buffer, pH 8) was hydrolysed in a flow-through, miniature electro-membrane reactor with the penicillin G acylase immobilized in 5% (w/v) polyacrylamide (diam. 10 mm, thickness 2.6 mm, enzyme activity 24 U ml(-1)). The conversion of penicillin G increased from 0.15 to almost 0.5 when the electric current applied to the reactor was changed from -600 to + 600 A/m(2) with a substrate residency of 1 h.

引用

页码：485 / 490

页数：6

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