Re-examination of resistance of Toxoplasma gondii tachyzoites and bradyzoites to pepsin and trypsin digestion

The effect of digestion in trypsin and acid pepsin on Toxoplasma gondii tachyzoites and bradyzoites was re-evaluated because of recent use of this method to distinguish tachyzoites from bradyzoites. Toxoplasma gondii tachyzoites survived better in 0.5% trypsin solution for 1 h than in 1.0% solution, and occasionally survived for 2 h in acid pepsin solution. Extracellular tachyzoites (greater than or equal to 1000) were also infectious orally to mice and cats. Bradyzoites survived equally in trypsin and acid pepsin solutions but the digestion of brain tissue in 0.5% trypsin solution was better than in acid pepsin solution. The resistance to digestion in acid pepsin solution is not a reliable method to distinguish tachyzoites from bradyzoites.