Mutational analysis of genes encoding the early flagellar components of Helicobacter pylori:: Evidence for transcriptional regulation of flagellin A biosynthesis

被引:31
作者
Allan, E [1 ]
Dorrell, N [1 ]
Foynes, S [1 ]
Anyim, M [1 ]
Wren, BW [1 ]
机构
[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Med, Pathogen Mol Biol & Biochem Unit, London WC1E 7HT, England
关键词
D O I
10.1128/JB.182.18.5274-5277.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We investigated the roles of fliF, fliS, flhB, fliQ, fliG, and fliI of Helicobacter pylori, predicted by homology to encode structural components of the flagellar basal body and export apparatus. Mutation of these genes resulted in nonmotile, nonflagellate strains. Western blot analysis showed that all the mutants had considerably reduced levels of both flagellin subunits and of FlgE, the flagellar hook protein. RNA slot blot hybridization showed reduced levels of flaA mRNA, indicating that transcription of the major flagellin gene is inhibited in the absence of the early components of the flagellar-assembly pathway. This is the first demonstration of a checkpoint in H. pylori flagellar assembly.
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页码:5274 / 5277
页数:4
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