Proteomics reveals novel components of the Anopheles gambiae eggshell

被引：52

作者：

Amenya, Dolphine A. ^{[1
,2
]}

Chou, Wayne ^{[1
]}

Li, Jianyong ^{[3
]}

Yan, Guiyun ^{[2
]}

Gershon, Paul D. ^{[1
]}

James, Anthony A. ^{[1
,4
]}

Marinotti, Osvaldo ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Program Publ Hlth, Irvine, CA 92697 USA

[3] Virginia Tech, Dept Biochem, Blacksburg, VA 24061 USA

[4] Univ Calif Irvine, Dept Microbiol & Mol Genet, Irvine, CA 92697 USA

来源：

JOURNAL OF INSECT PHYSIOLOGY | 2010年 / 56卷 / 10期

关键词：

Chorion; Vitelline membrane; OBPs; Phenoloxidase; Peroxidase; Mosquito; DROSOPHILA-MELANOGASTER; BINDING PROTEIN; MOSQUITO; CHORION; GENES; EVOLUTION; SURVIVAL; CLONES; CDNA;

D O I：

10.1016/j.jinsphys.2010.04.013

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

While genome and transcriptome sequencing has revealed a large number and diversity of Anopheles gambiae predicted proteins, identifying their functions and biosynthetic pathways remains challenging Applied mass spectrometry-based proteomics in conjunction with mosquito genome and transcriptome databases were used to identify 44 proteins as putative components of the eggshell. Among the identified molecules are two vitelline membrane proteins and a group of seven putative chorion proteins Enzymes with peroxidase, laccase and phenoloxidase activities, likely involved in cross-linking reactions that stabilize the eggshell structure, also were identified. Seven odorant binding proteins were found in association with the mosquito eggshell, although their role has yet to be demonstrated This analysis fills a considerable gap of knowledge about proteins that build the eggshell of anopheline mosquitoes. (C) 2010 Elsevier Ltd. All rights reserved

引用

页码：1414 / 1419

页数：6

共 35 条

[1]

Andrenacci D, 2001, J CELL SCI, V114, P2819

[2]

BEIER JC, 1990, J AM MOSQUITO CONTR, V6, P105

[3] Feature-based prediction of non-classical and leaderless protein secretion [J].

Bendtsen, JD ;

Jensen, LJ ;

Blom, N ;

von Heijne, G ;

Brunak, S .

PROTEIN ENGINEERING DESIGN & SELECTION, 2004, 17 (04) :349-356

[4] Isolation of cDNA clones encoding putative odourant binding proteins from the antennae of the malaria-transmitting mosquito, Anopheles gambiae [J].

Biessmann, H ;

Walter, MF ;

Dimitratos, S ;

Woods, D .

INSECT MOLECULAR BIOLOGY, 2002, 11 (02) :123-132

[5] Multifunctionality and mechanism of ligand binding in a mosquito antiinflammatory protein [J].

Calvo, Eric ;

Mans, Ben J. ;

Ribeiro, Jose M. C. ;

Andersen, John F. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (10) :3728-3733

[6] Building up the Drosophila eggshell:: First of all the eggshell genes must be transcribed [J].

Cavaliere, Valeria ;

Bernardi, Fabio ;

Romani, Patrizia ;

Duchi, Serena ;

Gargiulo, Giuseppe .

DEVELOPMENTAL DYNAMICS, 2008, 237 (08) :2061-2072

[7]

Christophers S.R., 1960, AEDES AEGYPTI L YELL, P738

[8] Gene amplification as a developmental strategy:: Isolation of two developmental amplicons in Drosophila [J].

Claycomb, JM ;

Benasutti, M ;

Bosco, G ;

Fenger, DD ;

Orr-Weaver, TL .

DEVELOPMENTAL CELL, 2004, 6 (01) :145-155

[9]

Deane M. P., 1943, AMER JOUR TROP MED, V23, P95

[10] Vitelline envelope genes of the yellow fever mosquito, Aedes aegypti [J].

Edwards, MJ ;

Severson, DW ;

Hagedorn, HH .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1998, 28 (12) :915-925

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