Nano-Bio-Chip Sensor Platform for Examination of Oral Exfoliative Cytology

被引:57
作者
Weigum, Shannon E. [1 ,2 ]
Floriano, Pierre N. [1 ,2 ]
Redding, Spencer W. [5 ]
Yeh, Chih-Ko [5 ]
Westbrook, Stephen D. [5 ]
McGuff, H. Stan [6 ]
Lin, Alan [5 ]
Miller, Frank R. [7 ]
Villarreal, Fred [7 ]
Rowan, Stephanie D. [7 ]
Vigneswaran, Nadarajah [3 ]
Williams, Michelle D. [4 ]
McDevitt, John T. [1 ,2 ]
机构
[1] Rice Univ, Dept Chem, Houston, TX 77005 USA
[2] Rice Univ, Dept Bioengn, Houston, TX 77005 USA
[3] Univ Texas Hlth Sci Ctr Houston, Dept Diagnost Sci, Houston, TX USA
[4] Univ Texas MD Anderson Canc Ctr, Dept Pathol, Houston, TX 77030 USA
[5] Univ Texas Hlth Sci Ctr San Antonio, Dept Dent Diagnost Sci, San Antonio, TX 78229 USA
[6] Univ Texas Hlth Sci Ctr San Antonio, Dept Pathol, San Antonio, TX 78229 USA
[7] Univ Texas Hlth Sci Ctr San Antonio, Dept Otolaryngol Head & Neck Surg, San Antonio, TX 78229 USA
基金
美国国家卫生研究院;
关键词
SQUAMOUS-CELL CARCINOMA; PREMALIGNANT LESIONS; BRUSH BIOPSY; NECK-CANCER; HEAD; LEUKOPLAKIA; EXPRESSION; RNA;
D O I
10.1158/1940-6207.CAPR-09-0139
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Oral cancer is a deadly and disfiguring disease that could greatly benefit from new diagnostic approaches enabling early detection. In this pilot study, we describe a nano-bio-chip (NBC) sensor technique for analysis of oral cancer biomarkers in exfoliative cytology specimens, targeting both biochemical and morphologic changes associated with early oral tumorigenesis. Here, oral lesions from 41 dental patients, along with normal epithelium from 11 healthy volunteers, were sampled using a non-invasive brush biopsy technique. Specimens were enriched, immunolabeled, and imaged in the NBC sensor according to previously established assays for the epidermal growth factor receptor (EGFR) biomarker and cytomorphometry. A total of 51 measurement parameters were extracted using custom image analysis macros, including EGFR labeling intensity, cell and nuclear size, and the nuclear-to-cytoplasmic ratio. Four key parameters were significantly elevated in both dysplastic and malignant lesions relative to healthy oral epithelium, including the nuclear area and diameter (P < 0.0001), the nuclear-to-cytoplasmic ratio (P < 0.0001), and EGFR biomarker expression (P < 0.03). Further examination using logistic regression and receiver operating characteristic curve analyses identified morphologic features as the best predictors of disease (area under the curve <= 0.93) individually, whereas a combination of all features further enhanced discrimination of oral cancer and precancerous conditions (area under the curve, 0.94) with high sensitivity and specificity. Further clinical trials are necessary to validate the regression model and evaluate other potential biomarkers, but this pilot study supports the NBC sensor technique as a promising new diagnostic tool for early detection of oral cancer, which could enhance patient care and survival. Cancer Prev Res; 3(4); 518-28. (C)2010 AACR.
引用
收藏
页码:518 / 528
页数:11
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