Differential expressions of antioxidant status in aging rats: the role of transcriptional factor Nrf2 and MAPK signaling pathway

被引：156

作者：

Shih, Ping-Hsiao ^{[1
]}

Yen, Gow-Chin ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, Taichung 40227, Taiwan

来源：

BIOGERONTOLOGY | 2007年 / 8卷 / 02期

关键词：

oxidative stress; antioxidant enzyme; aging; signal transduction; mitogen-activated protein kinase (MAPK); nuclear factor-erythroid 2 p45-related factor 2 (Nrf2);

D O I：

10.1007/s10522-006-9033-y

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Antioxidant enzymes (AOEs) play an important role in the protection of cells against reactive oxygen species and facilitate the prevention of oxidative stress-induced aging. In the present study, the antioxidant indices, including the content of peroxidation product and the expression of AOEs in rat livers of varying ages (2, 12 and 18-24 months old) were evaluated. Erythrocytes haemolysis induced by free radicals showed significant age-dependent increases (P < 0.05). The content of oxidation products in livers showed that increasing age was associated with serious oxidative injury. The activities of AOEs decreased with increasing age. Expression of the antioxidant and age-related gene, klotho, decreased with increasing age. Western blot assay showed that aged rats experience higher levels of oxidative stress. Nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) exhibited an age-dependent decrease. Additionally, the mitogen-activated protein kinase cascade (MAPK) played a regulatory role in signaling transduction. Overall, we suggest that age-related declines of the antioxidant defense are closely involved with the expression of Nrf2 and are regulated by the MAPK family.

引用

页码：71 / 80

页数：10

共 28 条

[1] AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2] BLOCK G, 2003, CRITICAL REV OXIDATI, P870
[3] Buege J A, 1978, Methods Enzymol, V52, P302
[4] Age-related changes in superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea
Cejková, J
Vejrazka, M
Pláteník, J
Stípek, S
[J]. EXPERIMENTAL GERONTOLOGY, 2004, 39 (10) : 1537 - 1543
[5] Induction of detoxifying enzymes by garlic organosulfur compounds through transcription factor Nrf2: Effect of chemical structure and stress signals
Chen, C
Pung, D
Leong, V
Hebbar, V
Shen, GX
Nair, S
Li, W
Kong, ANT
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 2004, 37 (10) : 1578 - 1590
[6] MAPK pathways in radiation responses
Dent, P
Yacoub, A
Fisher, PB
Hagan, MP
Grant, S
[J]. ONCOGENE, 2003, 22 (37) : 5885 - 5896
[7] Devasagayam T P A, 2004, J Assoc Physicians India, V52, P794
[8] The Nrf2 transcription factor contributes both to the basal expression of glutathione S-transferases in mouse liver and to their induction by the chemopreventive synthetic antioxidants, butylated hydroxyanisole and ethoxyquin
Hayes, JD
Chanas, SA
Henderson, CJ
McMahon, M
Sun, C
Moffat, GJ
Wolf, CR
Yamamoto, M
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2000, 28 : 33 - 41
[9] Carnosine, the anti-ageing, anti-oxidant dipeptide, may react with protein carbonyl groups
Hipkiss, AR
Brownson, C
Carrier, MJ
[J]. MECHANISMS OF AGEING AND DEVELOPMENT, 2001, 122 (13) : 1431 - 1445
[10] JAISWAL AK, 1988, J BIOL CHEM, V263, P13572

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