Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine

被引：48

作者：

Gammelgaard, B ^{[1
]}

Cornett, C ^{[1
]}

Olsen, J ^{[1
]}

Bendahl, L ^{[1
]}

Hansen, SH ^{[1
]}

机构：

[1] Royal Danish Sch Pharm, Dept Analyt Chem, DK-2100 Copenhagen, Denmark

来源：

TALANTA | 2003年 / 59卷 / 06期

关键词：

selenomethionine; selenoxide; selenium speciation;

D O I：

10.1016/S0039-9140(03)00026-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Selenomethionine (SeMet) was oxidized by heating an acidic solution with hydrogen peroxide. Samples were taken before and during the oxidation process. The oxidation products were separated by cation exchange chromatography followed by ICP-MS detection to identify the selenium containing compounds as well as electrospray ionization MS detection to determine the masses of the degradation products. Furthermore, the samples were analyzed by Se-77-NMR. The first appearing degradation product was selenomethionine selenoxide, which was converted via the deaminated selenoxide to methane seleninic acid and selenite. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：1165 / 1171

页数：7

共 11 条

[1]

[Anonymous], 1986, CHEM ORGANIC SELENIU

[2]

KLAPOTKE TM, 1996, COMPILATION RECORDED

[3]

Klayman D.L., 1973, ORGANIC SELENIUM COM

[4] High-performance liquid chromatography of selenium compounds utilizing perfluorinated carboxylic acid ion-pairing agents and inductively coupled plasma and electrospray ionization mass spectrometric detection [J].

Kotrebai, M ;

Tyson, JF ;

Block, E ;

Uden, PC .

JOURNAL OF CHROMATOGRAPHY A, 2000, 866 (01) :51-63

[5] Speciation of selenoamino acids, selenonium ions and inorganic selenium by ion exchange HPLC with mass spectrometric detection and its application to yeast and algae [J].

Larsen, EH ;

Hansen, M ;

Fan, T ;

Vahl, M .

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2001, 16 (12) :1403-1408

[6] UNTERSUCHUNGEN AN SELEN - SAUERSTOFF-VERBINDUNGEN .41. DIPHENYLSELENDIALKOXIDE (C6H5)2SE(OR)2 [J].

PAETZOLD, R ;

LINDNER, U .

ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE, 1967, 350 (5-6) :295-&

[7] The importance of selenium to human health [J].

Rayman, MP .

LANCET, 2000, 356 (9225) :233-241

[8] Analytical selenoamino acid studies by chromatography with interfaced atomic mass spectrometry and atomic emission spectral detection [J].

Uden, PC ;

Bird, SM ;

Kotrebai, M ;

Nolibos, P ;

Tyson, JF ;

Block, E ;

Denoyer, E .

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1998, 362 (05) :447-456

[9]

Zainal HA, 1998, J CHEM TECHNOL BIOT, V72, P38, DOI 10.1002/(SICI)1097-4660(199805)72:1<38::AID-JCTB863>3.3.CO

[10]

2-I

← 1 2 →