MR microscopy of magnetically labeled neurospheres transplanted into the Lewis EAE rat brain

被引：107

作者：

Bulte, JWM

Ben-Hur, T

Miller, BR

Mizrachi-Kol, R

Einstein, O

Reinhartz, E

Zywicke, HA

Douglas, T

Frank, JA

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Radiol & Radiol Sci, Baltimore, MD 21205 USA

[2] NIH, Ctr Clin, Expt Neuroimaging Sect, Bethesda, MD USA

[3] Hadassah Univ Hosp, Agnes Ginges Ctr Human Neurogenet, Dept Neurol, IL-91120 Jerusalem, Israel

[4] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA

来源：

MAGNETIC RESONANCE IN MEDICINE | 2003年 / 50卷 / 01期

关键词：

MR microscopy; transplantation; iron oxide MR contrast agent; neurospheres; experimental autoimmune encephalomyelitis;

D O I：

10.1002/mrm.10511

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Stem cell transplantation is being explored as a new paradigm for the treatment of demyelinating diseases. Magnetically labeled multipotential neural precursor cells were transplanted into the ventricles of rats with acute experimental allergic encephalomyelitis (EAE) and high-resolution (microscopic) MR images were obtained ex vivo. Migration patterns of live cells into periventricular white matter structures could be easily visualized, with a good correlation of the corresponding histopathology. The present results confirm that MR cell tracking can be used to guide the development of successful transplantation protocols. (C) 2003 Wiley-Liss, Inc.

引用

收藏

页码：201 / 205

页数：5

相关论文

共 21 条

[1] Myelination of the canine central nervous system by glial cell transplantation: A model for repair of human myelin disease [J].

Archer, DR ;

Cuddon, PA ;

Lipsitz, D ;

Duncan, ID .

NATURE MEDICINE, 1997, 3 (01) :54-59

[2] HOECHST-33342 A SUITABLE FLUORESCENT MARKER FOR SCHWANN-CELLS AFTER TRANSPLANTATION IN THE MOUSE SPINAL-CORD [J].

BARONVANEVERCOOREN, A ;

GANSMULLER, A ;

CLERIN, E ;

GUMPEL, M .

NEUROSCIENCE LETTERS, 1991, 131 (02) :241-244

[3] Transplanted multipotential neural precursor cells migrate into the inflamed white matter in response to experimental autoimmune encephalomyelitis [J].

Ben-Hur, T ;

Einstein, O ;

Mizrachi-Kol, R ;

Ben-Menachem, O ;

Reinhartz, E ;

Karussis, D ;

Abramsky, O .

GLIA, 2003, 41 (01) :73-80

[4] Growth and fate of PSA-NCAM+ precursors of the postnatal brain [J].

Ben-Hur, T ;

Rogister, B ;

Murray, K ;

Rougon, G ;

Dubois-Dalcq, M .

JOURNAL OF NEUROSCIENCE, 1998, 18 (15) :5777-5788

[5] TRANSPLANTATION OF GLIAL-CELLS INTO THE CNS [J].

BLAKEMORE, WF ;

FRANKLIN, RJM .

TRENDS IN NEUROSCIENCES, 1991, 14 (08) :323-327

[6] Embryonic stem cell-derived glial precursors:: A source of myelinating transplants [J].

Brüstle, O ;

Jones, KN ;

Learish, RD ;

Karram, K ;

Choudhary, K ;

Wiestler, OD ;

Duncan, ID ;

McKay, RDG .

SCIENCE, 1999, 285 (5428) :754-756

[7] Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells [J].

Bulte, JWM ;

Douglas, T ;

Witwer, B ;

Zhang, SC ;

Strable, E ;

Lewis, BK ;

Zywicke, H ;

Miller, B ;

van Gelderen, P ;

Moskowitz, BM ;

Duncan, ID ;

Frank, JA .

NATURE BIOTECHNOLOGY, 2001, 19 (12) :1141-1147

[8] Neurotransplantation of magnetically labeled oligodendrocyte progenitors: Magnetic resonance tracking of cell migration and myelination [J].

Bulte, JWM ;

Zhang, SC ;

van Gelderen, P ;

Herynek, V ;

Jordan, EK ;

Duncan, ID ;

Frank, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :15256-15261

[9] O2A PROGENITOR CELLS TRANSPLANTED INTO THE NEONATAL RAT-BRAIN DEVELOP INTO OLIGODENDROCYTES BUT NOT ASTROCYTES [J].

DELOSMONTEROS, AE ;

ZHANG, MS ;

DEVELLIS, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (01) :50-54

[10] Magnetic resonance imaging of transplanted oligodendrocyte precursors in the rat brain [J].

Franklin, RJM ;

Blaschuk, KL ;

Bearchell, MC ;

Prestoz, LLC ;

Setzu, A ;

Brindle, KM ;

ffrench-Constant, C .

NEUROREPORT, 1999, 10 (18) :3961-3965