Codon-based mutagenesis using dimer-phosphoramidites

被引：29

作者：

Neuner, P

Cortese, R

Monaci, P

机构：

[1] Ist Ric Biol Mol P Angeletti, Dept Chem, I-00040 Rome, Italy

[2] Ist Ric Biol Mol P Angeletti, Dept Biotechnol, I-00040 Rome, Italy

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 05期

关键词：

D O I：

10.1093/nar/26.5.1223

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new approach for the synthesis of randomized DNA sequences containing the 20 codons corresponding to all natural amino acids is described, The strategy is based on the use of dinucleotide phosphoramidite building blocks within a resin-splitting procedure. Through this protocol, a minimal number of seven dimers is sufficient to encode all 20 natural amino acids, This synthesis procedure is extremely flexible and allows codon usage from different hosts to be accommodated.

引用

页码：1223 / 1227

页数：5

共 15 条

[1] Construction of a semisynthetic antibody library using trinucleotide oligos
Braunagel, M
Little, M
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (22) : 4690 - 4691
[2] BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376
[3] REGIONAL CODON RANDOMIZATION - DEFINING A TATA-BINDING PROTEIN SURFACE REQUIRED FOR RNA POLYMERASE-III TRANSCRIPTION
CORMACK, BP
STRUHL, K
[J]. SCIENCE, 1993, 262 (5131) : 244 - 248
[4] GLASER SM, 1992, J IMMUNOL, V149, P3903
[5] IDENTIFICATION OF BIOLOGICALLY-ACTIVE MUTANTS BY COMBINATORIAL CASSETTE MUTAGENESIS - EXCLUSION OF WILD-TYPE CODON FROM DEGENERATE CODONS
HUANG, WD
SANTI, DV
[J]. ANALYTICAL BIOCHEMISTRY, 1994, 218 (02) : 454 - 457
[6] HUSE WD, 1993, Patent No. 5264563
[7] A convenient approach to the synthesis of trinucleotide phosphoramidites - Synthons for the generation of oligonucleotide/peptide libraries
Kayushin, AL
Korosteleva, MD
Miroshnikov, AI
Kosch, W
Zubov, D
Piel, N
[J]. NUCLEIC ACIDS RESEARCH, 1996, 24 (19) : 3748 - 3755
[8] IMPROVEMENTS IN OLIGODEOXYRIBONUCLEOTIDE SYNTHESIS - METHYL N,N-DIALKYLPHOSPHORAMIDITE DIMER UNITS FOR SOLID SUPPORT PHOSPHITE METHODOLOGY
KUMAR, G
POONIAN, MS
[J]. JOURNAL OF ORGANIC CHEMISTRY, 1984, 49 (25) : 4905 - 4912
[9] A NEW TYPE OF SYNTHETIC PEPTIDE LIBRARY FOR IDENTIFYING LIGAND-BINDING ACTIVITY
LAM, KS
SALMON, SE
HERSH, EM
HRUBY, VJ
KAZMIERSKI, WM
KNAPP, RJ
[J]. NATURE, 1991, 354 (6348) : 82 - 84
[10] LYTTLE MH, 1995, BIOTECHNIQUES, V19, P274

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