Function of the σE regulon in dead-cell lysis in stationary-phase Escherichia coli

被引:42
作者
Nitta, T [1 ]
Nagamitsu, H [1 ]
Murata, M [1 ]
Izu, H [1 ]
Yamada, M [1 ]
机构
[1] Yamaguchi Univ, Fac Agr, Dept Biol Chem, Yamaguchi 7538515, Japan
关键词
D O I
10.1128/JB.182.18.5231-5237.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Elevation of active sigma(E) levels in Escherichia coli by either repressing the expression of rseA encoding an anti-sigma(E) factor or cloning rpoE in a multicopy plasmid, led to a large decrease in the number of dead cells and the accumulation of cellular proteins in the medium in the stationary phase. The numbers of CFU, however, were nearly the same as those of the wild type or cells devoid of the cloned gene. In the wild-type cells, rpoE expression was increased in the stationary phase and a low-level release of intracellular proteins was observed. These results suggest that dead fell lysis in stationary-phase E. coli occurs in a sigma(E)-dependent fashion. We propose there is a novel physiological function of the sigma(E) regulon that may guarantee cell survival in prolonged stationary phase by providing nutrients from dead cells for the next generation.
引用
收藏
页码:5231 / 5237
页数:7
相关论文
共 26 条
[1]   Evidence that rseC, a gene in the rpoE cluster, has a role in thiamine synthesis in Salmonella typhimurium [J].
Beck, BJ ;
Connolly, LE ;
DelasPenas, A ;
Downs, DM .
JOURNAL OF BACTERIOLOGY, 1997, 179 (20) :6504-6508
[2]   CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].
BOLIVAR, F ;
RODRIGUEZ, RL ;
GREENE, PJ ;
BETLACH, MC ;
HEYNEKER, HL ;
BOYER, HW ;
CROSA, JH ;
FALKOW, S .
GENE, 1977, 2 (02) :95-113
[3]   CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].
CHANG, ACY ;
COHEN, SN .
JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156
[4]   The sigma(E) and the Cpx signal transduction systems control the synthesis of periplasmic protein-folding enzymes in Escherichia coli [J].
Danese, PN ;
Silhavy, TJ .
GENES & DEVELOPMENT, 1997, 11 (09) :1183-1193
[5]   The sigma(E)-mediated response to extracytoplasmic stress in Escherichia coli is transduced by RseA and RseB, two negative regulators of sigma(E) [J].
DeLasPenas, A ;
Connolly, L ;
Gross, CA .
MOLECULAR MICROBIOLOGY, 1997, 24 (02) :373-385
[6]   IDENTIFICATION OF THE SIGMA-E SUBUNIT OF ESCHERICHIA-COLI RNA-POLYMERASE - A 2ND ALTERNATE SIGMA-FACTOR INVOLVED IN HIGH-TEMPERATURE GENE-EXPRESSION [J].
ERICKSON, JW ;
GROSS, CA .
GENES & DEVELOPMENT, 1989, 3 (09) :1462-1471
[7]   THE RPOE GENE OF ESCHERICHIA-COLI, WHICH ENCODES SIGMA(E), IS ESSENTIAL FOR BACTERIAL-GROWTH AT HIGH-TEMPERATURE [J].
HIRATSU, K ;
AMEMURA, M ;
NASHIMOTO, H ;
SHINAGAWA, H ;
MAKINO, K .
JOURNAL OF BACTERIOLOGY, 1995, 177 (10) :2918-2922
[8]  
KLECKNER N, 1991, METHOD ENZYMOL, V204, P139
[9]   THE PHYSICAL MAP OF THE WHOLE ESCHERICHIA-COLI CHROMOSOME - APPLICATION OF A NEW STRATEGY FOR RAPID ANALYSIS AND SORTING OF A LARGE GENOMIC LIBRARY [J].
KOHARA, Y ;
AKIYAMA, K ;
ISONO, K .
CELL, 1987, 50 (03) :495-508
[10]   THE STATIONARY-PHASE OF THE BACTERIAL LIFE-CYCLE [J].
KOLTER, R ;
SIEGELE, DA ;
TORMO, A .
ANNUAL REVIEW OF MICROBIOLOGY, 1993, 47 :855-874