Construction and characterization of nitrate reductase-based amperometric electrode and nitrate assay of fertilizers and drinking water

被引：68

作者：

Glazier, SA ^{[1
]}

Campbell, ER

Campbell, WH

机构：

[1] Natl Inst Stand & Technol, Div Biotechnol, Biomol Mat Grp, Gaithersburg, MD 20899 USA

[2] Nitrate Eliminat Co Inc, Lake Linden, MI 49945 USA

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 08期

关键词：

D O I：

10.1021/ac971146s

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The construction and characterization of a nitrate reductase-based amperometric electrode for determination of nitrate ion is described. The electrode consisted of nitrate reductase held by dialysis membrane onto a Nation-coated glassy carbon electrode. Methyl viologen was allowed to absorb into the Nation layer, which acted as a reservoir for the electron mediator. The utility of the electrode to assay fertilizer and water sample for nitrate was demonstrated. The assays conducted with this electrode compared well with colorimetric and potentiometric assays of the same samples.

引用

页码：1511 / 1515

页数：5

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