Reduced glycosaminoglycan sulfation diminishes the agrin signal transduction pathway

被引:11
作者
McDonnell, KMW [1 ]
Grow, WA [1 ]
机构
[1] Midwestern Univ, Arizona Coll Osteopath Med, Dept Anat, Glendale, AZ 85308 USA
关键词
acetylcholine receptor; agrin; C2C12; chlorate; glycosaminoglycan; neuromuscular synapse; proteoglycan;
D O I
10.1159/000080706
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Proteoglycans consist of a protein core complexed to glycosaminoglycan (GAG) side chains, are abundant in skeletal muscle cell membranes and basal lamina, and have important functions in neuromuscular synapse development. Treatment with chlorate results in the undersulfation of heparan sulfate and chondroitin sulfate GAGs in cell culture. In addition, chlorate treatment decreases the frequency of spontaneous acetylcholine receptor (AChR) clustering in skeletal muscle cell culture. AChRs and other molecules cluster to form the postsynaptic component of neuromuscular synapses. Chlorate treatment is shown here to decrease the frequency of agrin-induced AChR clustering and agrin-induced tyrosine phosphorylation of the AChR beta-subunit. These data suggest that reduced GAG chain sulfation decreases the frequency of AChR clustering by diminishing the agrin signal transduction pathway. Copyright (C) 2004 S. Karger AG, Basel.
引用
收藏
页码:1 / 10
页数:10
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