An inexpensive and high-throughput procedure to extract and purify total genomic DNA for population studies

被引：260

作者：

Elphinstone, MS

Hinten, GN

Anderson, MJ

Nock, CJ

机构：

[1] Univ Queensland, Princess Alexandra Hosp, Dept Med, Wollongabba, Qld 4102, Australia

[2] So Cross Univ, Ctr Anim Conservat Genet, Lismore, NSW 2480, Australia

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 02期

关键词：

DNA; genomic; population; purification; rapid; silica;

D O I：

10.1046/j.1471-8286.2003.00397.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here a procedure for the purification of high molecular weight genomic DNA that combines the economies of 'do-it-yourself', single-tube protocols with the sample throughput and DNA quality of microplate-based DNA extraction and purification kits. from commercial suppliers. The procedure allows the preparation of genomic DNA of a quality suitable for polymerase chain reaction-based studies of large populations at around one-tenth of the cost of commercially available kits. Furthermore, 96 samples can be purified from crude tissue digests in around 30 min and are produced in microtitre plate format to allow efficient downstream processing of samples.

引用

页码：317 / 320

页数：4

共 15 条

[1]

Baker LE, 2001, J FORENSIC SCI, V46, P126

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