Preparing polyA-containing RNA internal standards for multiplex competitive RT-PCR

被引：4

作者：

Celi, FS ^{[1
]}

Mentuccia, D ^{[1
]}

Proietti-Pannunzi, L ^{[1
]}

di Gioia, CRT ^{[1
]}

Andreoli, M ^{[1
]}

机构：

[1] Univ Rome, Dipartimento Med Sperimentale & Patol, Cattedra Endocrinol 1, I-00161 Rome, Italy

来源：

BIOTECHNIQUES | 2000年 / 29卷 / 03期

关键词：

D O I：

10.2144/00293bm09

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：454 / +

页数：3

共 12 条

[1] Quantitation of gene expression by means of HPLC analysis of RT-PCR products [J].

Bachmair, F ;

Huber, C ;

Daxenbichler, G .

CLINICA CHIMICA ACTA, 1999, 279 (1-2) :25-34

[2] Direct quantitation of RNA transcripts by competitive single-tube RT-PCR and capillary electrophoresis [J].

Borson, ND ;

Strausbauch, MA ;

Wettstein, PJ ;

Oda, RP ;

Johnston, SL ;

Landers, JP .

BIOTECHNIQUES, 1998, 25 (01) :130-+

[3] A RAPID AND VERSATILE METHOD TO SYNTHESIZE INTERNAL STANDARDS FOR COMPETITIVE PCR [J].

CELI, FS ;

ZENILMAN, ME ;

SHULDINER, AR .

NUCLEIC ACIDS RESEARCH, 1993, 21 (04) :1047-1047

[4] Quantitation of messenger RNA by competitive RT-PCR: a simplified read out assay [J].

Colle, JH ;

Falanga, PB ;

Singer, M ;

Hevin, B ;

Milon, G .

JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 210 (02) :175-184

[5] Quantitative RT-PCR: Pitfalls and potential [J].

Freeman, WM ;

Walker, SJ ;

Vrana, KE .

BIOTECHNIQUES, 1999, 26 (01) :112-+

[6] Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA [J].

Hall, LL ;

Bicknell, GR ;

Primrose, L ;

Pringle, JH ;

Shaw, JA ;

Furness, PN .

BIOTECHNIQUES, 1998, 24 (04) :652-+

[7]

Kobayashi H, 1997, J CLIN LAB ANAL, V11, P258, DOI 10.1002/(SICI)1098-2825(1997)11:5<258::AID-JCLA4>3.3.CO

[8]

2-Y

[9] A synthetic standard for competitive RT-PCR quantitation of 13 GABA receptor type A subunit mRNAs in rats and mice [J].

Liu, ZF ;

Burt, DR .

JOURNAL OF NEUROSCIENCE METHODS, 1998, 85 (01) :89-98

[10] Genetic structure of the first brood of workers and mating frequency of queens in a Japanese paper wasp, Polistes chinensis antennalis [J].

Miyano, S ;

Hasegawa, E .

ETHOLOGY ECOLOGY & EVOLUTION, 1998, 10 (01) :79-85

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