Transgenic apple (Malus x domestica) shoot showing low browning potential

被引：29

作者：

Murata, M

Haruta, M

Murai, N

Tanikawa, N

Nishimura, M

Homma, S

Itoh, A

机构：

[1] Ochanomizu Univ, Dept Nutr & Food Sci, Bunkyo Ku, Tokyo 1128610, Japan

[2] Natl Inst Agrobiol Resources, Omiya, Saitama 31922, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 11期

关键词：

apple (Malus x domestica); enzymatic browning; polyphenol oxidase; antisense; transgenic plant; Agrobacterium transformation;

D O I：

10.1021/jf000771m

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Transgenic apple shoots were prepared from leaf disks by using Agrobacterium tumefaciens carrying the kanamycin (KM) resistance gene and antisense polyphenol oxidase (PPO) DNA. Four transgenic apple lines that grew on the medium containing 50 mug/mL KM were obtained. They contained the KM resistance gene and grew stably on the medium for >3 years. Two transgenic shoot lines containing antisense PPO DNA in which PPO activity was repressed showed a lower browning potential than a control shoot.

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收藏

页码：5243 / 5248

页数：6

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