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Kinetic spectrofluorimetric determination of trace ascorbic acid based on its inhibition on the oxidation of pyronine Y by nitrite

被引：13
作者：
Feng, SL
Wang, J
Chen, XG [1 ]
Fan, J
机构：
[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
[2] Henan Normal Univ, Coll Chem & Environm Sci, Xinxiang 453007, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2005年 / 61卷 / 05期
关键词：
ascorbic acid; kinetic spectrofluorimetry; inhibiton; pyronine Y;
D O I：
10.1016/j.saa.2004.06.008
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A highly sensitive spectrofluorimetric method is proposed for the determination of trace amount of ascorbic acid using a new indication. The method is based on the inhibition of ascorbic acid on the oxidation of pyronine Y (PRY) by nitrite. The detection limit for ascorbic acid is 0.012 mug ml(-1), the linear range of the determination is 0.02-0.36 mug ml(-1). Analytical parameters, such as reagent concentration, pH, reaction temperature and time, were optimized. The relative standard deviations of eleven replication determinations of 0.12 and 0.24 mug ml(-1) ascorbic acid were 1.4 and 0.72%, respectively. This method has been used to determine ascorbic acid in pharmaceuticals, vegetables, fruits and soft drink with satisfactory results. (C) 2004 Elsevier B.V. All rights reserved.
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收藏
页码：841 / 844
页数：4
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