First isolation and molecular characterization of Ehrlichia canis in Spain

被引：48

作者：

Aguirre, E

Sainz, A ^{[1
]}

Dunner, S

Amusategui, I

López, L

Rodríguez-Franco, F

Luaces, I

Cortés, O

Tesouro, MA

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Med & Cirugia Anim, E-28040 Madrid, Spain

[2] Univ Complutense Madrid, Fac Vet, Dept Anim Prod, E-28040 Madrid, Spain

[3] Inmunol & Genet Aplicada SA, Madrid 28037, Spain

[4] Fac Vet, Dept Pathol Anim Med Vet, Leon 24071, Spain

来源：

VETERINARY PARASITOLOGY | 2004年 / 125卷 / 3-4期

关键词：

Ehrlichia canis; dog; Spain; isolation; sequence;

D O I：

10.1016/j.vetpar.2004.08.007

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学]; 100103 [病原生物学];

摘要：

This paper reports the first isolation and culture of Ehrlichia canis in Spain from a naturally infected dog using the DH82 cell line. After DNA extraction and PCR amplification, a nearly complete (1412 bp) sequence of the 16S rRNA gene of the new E. canis strain was obtained. The GenBank accession number for the nucleotide sequence of this strain is AY394465. This sequence was aligned with the 16S rRNA gene sequences of other Ehrlichia strains accessible in GenBank. The 16S rRNA gene sequence of the E. canis strain reported here showed a high percentage of similarity with the 16S rRNA gene sequence of E. canis from different geographic areas including Japan, Venezuela and Israel. These data confirm the presence of E. canis in Spain. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：365 / 372

页数：8

共 32 条

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