Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides

被引：28

作者：

Aussel, L

Chaby, R

Le Blay, K

Kelly, J

Thibault, PE

Perry, MB

Caroff, M ^{[1
]}

机构：

[1] Univ Paris 11, CNRS, UMR 8619, Equipe Endotoxines, F-91405 Orsay, France

[2] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

FEBS LETTERS | 2000年 / 485卷 / 01期

关键词：

endotoxin; structure; mass spectrometry; serology; Bordetella hinzii;

D O I：

10.1016/S0014-5793(00)02181-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) mere investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results: [GRAPHICS] Mass spectrometry and serology revealed that the O-chains mere different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and mere composed of a trisaccharide repeating unit. Masses up to 8 kDa mere obtained for native LPS molecular species. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：40 / 46

页数：7

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